Abstract
Cannabis has been used historically for both medicinal and recreational purposes, with the most notable cannabinoids being cannabidiol (CBD) and tetrahydrocannabinol (THC). Although their therapeutic effects have been well studied and their recreational use is highly debated, the underlying mechanisms of their biological effects remain poorly defined. In this study, we use isobaric tag-based sample multiplexed proteome profiling to investigate protein abundance differences in the human neuroblastoma SH-SY5Y cell line treated with CBD and THC. We identified significantly regulated proteins by each treatment and performed a pathway classification and associated protein–protein interaction analysis. Our findings suggest that these treatments may lead to mitochondrial dysfunction and induce endoplasmic reticulum stress. These data can potentially be interrogated further to investigate the potential role of CBD and THC in various biological and disease contexts, providing a foundation for future studies.
| Original language | English |
|---|---|
| Article number | 36 |
| Pages (from-to) | 1-20 |
| Number of pages | 20 |
| Journal | Proteomes |
| Volume | 11 |
| Issue number | 4 |
| Early online date | 2 Nov 2023 |
| DOIs | |
| Publication status | Published - Dec 2023 |
Bibliographical note
Copyright the Author(s) 2023. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.Keywords
- cannabidiol
- Delta-9-tetrahydrocannabinol
- endoplasmic reticulum stress
- high-field asymmetric ion mobility spectrometry
- mitochondrial dysfunction
- quantitative proteomics
- real-time database searching
- tandem mass tags
- TMTpro
- unfolded protein response