Proteomes of leaf-growing zones in rice genotypes with contrasting drought tolerance

Yunqi Wu, Mehdi Mirzaei, Dana Pascovici, Paul A. Haynes, Brian J. Atwell*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Plants require a distinctive cohort of enzymes to coordinate cell division and expansion. Proteomic analysis now enables interrogation of immature leaf bases where these processes occur. Hence, proteins in tissues sampled from leaves of a drought-tolerant rice (IAC1131) are investigated to provide insights into the effect of soil drying on gene expression relative to the drought-sensitive genotype Nipponbare. Shoot growth zones are dissected to estimate the proportion of dividing cells and extract protein for subsequent tandem mass tags quantitative proteomic analysis. Gene ontology annotations of differentially expressed proteins provide insights into responses of Nipponbare and IAC1131 to drought. Soil drying does not affect the percentage of mitotic cells in IAC1131. More than 800 proteins across most functional categories increase in drought (and decrease on rewatering) in IAC1131, including proteins involved in “organizing the meristem” and “new cell formation”. On the other hand, the percentage of dividing cells in Nipponbare is severely impaired during drought and fewer than 200 proteins respond in abundance when growing zones undergo a drying cycle. Remarkably, the proteomes of the growing zones of each genotype respond in a highly distinctive manner, reflecting their contrasting drought tolerance even at the earliest stages of leaf development.

Original languageEnglish
Article number1800310
Pages (from-to)1-11
Number of pages11
JournalProteomics
Volume19
Issue number9
DOIs
Publication statusPublished - May 2019

Keywords

  • abiotic stress
  • drought stress
  • growing zone
  • plant proteomics
  • rice

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