Proteomic analysis of a filamentous fungal endophyte using EST datasets

Shalome A. Bassett, Judy J. Bond, Fiona Y S Kwan, Alan F. McCulloch, Paul A. Haynes, Richard D. Johnson, Gregory T. Bryan, T. William Jordan

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Proteomic analysis of many species of fungi, particularly filamentous fungi, is difficult due to the lack of publicly available genome sequence data and the problems associated with cross-species comparisons. Furthermore, the detection of fungal proteins in biological systems where there are a greater number of proteins present from other eukaryote species provides additional challenges. We present an EST-based approach for identifying proteins from a fungal endophyte of temperate grasses and demonstrate that this method is well suited for fungi with minimal sequence data.

Original languageEnglish
Pages (from-to)2295-2300
Number of pages6
JournalProteomics
Volume9
Issue number8
DOIs
Publication statusPublished - Apr 2009

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