Proteomic analysis of Drosophila mojavensis male accessory glands suggests novel classes of seminal fluid proteins

Erin S. Kelleher*, Thomas D. Watts, Brooke A. LaFlamme, Paul A. Haynes, Therese A. Markow

*Corresponding author for this work

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Fruit-flies of the genus Drosophila are characterized by overwhelming variation in fertilization traits such as copulatory plug formation, sperm storage organ use, and nutritional ejaculatory donation. Despite extensive research on the genetic model Drosophila melanogaster, little is known about the molecular underpinnings of these interspecific differences. This study employs a proteomic approach to pin-point candidate seminal fluid proteins in Drosophila mojavensis, a cactophilic fruit-fly that exhibits divergent reproductive biology when compared to D. melanogaster. We identify several classes of candidate seminal fluid proteins not previously documented in the D. melanogaster male ejaculate, including metabolic enzymes, nutrient transport proteins, and clotting factors. Conversely, we also define 29 SFPs that are conserved despite >40 million years of Drosophila evolution. We discuss our results in terms of universal processes in insect reproduction, as well as the specialized reproductive biology of D. mojavensis.

Original languageEnglish
Pages (from-to)366-371
Number of pages6
JournalInsect Biochemistry and Molecular Biology
Volume39
Issue number5-6
DOIs
Publication statusPublished - May 2009

Fingerprint Dive into the research topics of 'Proteomic analysis of Drosophila mojavensis male accessory glands suggests novel classes of seminal fluid proteins'. Together they form a unique fingerprint.

Cite this