Proteomic analysis of the meristematic root zone in contrasting genotypes reveals new insights in drought tolerance in rice

Somayeh Abdirad, Yunqi Wu, Zahra Ghorbanzadeh, Sara Esmaeili Tazangi, Ardeshir Amirkhani, Matthew J. Fitzhenry, Mehrbano Kazemi, Mohammad Reza Ghaffari, Parisa Koobaz, Mehrshad Zeinalabedini, Fatemeh Habibpourmehraban, Farhad Masoomi-Aladizgeh, Brian J. Atwell, Mehdi Mirzaei, Ghasem Hosseini Salekdeh, Paul A. Haynes*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)
41 Downloads (Pure)

Abstract

Drought is responsible for major losses in rice production. Root tips contain meristematic and elongation zones that play major roles in determination of root traits and adaptive strategies to drought. In this study we analysed two contrasting genotypes of rice: IR64, a lowland, drought-susceptible, and shallow-rooting genotype; and Azucena, an upland, drought-tolerant, and deep-rooting genotype. Samples were collected of root tips of plants grown under control and water deficit stress conditions. Quantitative proteomics analysis resulted in the identification of 7294 proteins from the root tips of IR64 and 6307 proteins from Azucena. Data are available via ProteomeXchange with identifier PXD033343. Using a Partial Least Square Discriminant Analysis on 4170 differentially abundant proteins, 1138 statistically significant proteins across genotypes and conditions were detected. Twenty two enriched biological processes showing contrasting patterns between two genotypes in response to stress were detected through gene ontology enrichment analysis. This included identification of novel proteins involved in root elongation with specific expression patterns in Azucena, including four Expansins and seven Class III Peroxidases. We also detected an antioxidant network and a metallo-sulfur cluster assembly machinery in Azucena, with roles in reactive oxygen species and iron homeostasis, and positive effects on root cell cycle, growth and elongation.

Original languageEnglish
Article number2200100
Pages (from-to)1-16
Number of pages16
JournalProteomics
Volume22
Issue number21
Early online date21 Aug 2022
DOIs
Publication statusPublished - Nov 2022

Bibliographical note

Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

  • contrasting genotypes
  • drought
  • Oryza sativa
  • plant proteomics
  • rice
  • root tip
  • water-deficit stress

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