Proteomic and metabolomic analysis of desiccation tolerance in wheat young seedlings

Parisa Koobaz, Mohammad Reza Ghaffari, Manzar Heidari, Mehdi Mirzaei, Faezeh Ghanati, Ardeshir Amirkhani, Seyed Elyas Mortazavi, Foad Moradi, Mohammad Reza Hajirezaei, Ghasem Hosseini Salekdeh*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Young wheat seedlings are desiccation tolerant and have the capacity to withstand long dehydration period. In this study, we characterized the proteome and metabolome of wheat seedlings during desiccation and after recovery. Functional classification of differentially identified proteins revealed dynamic changes in the number and abundance of proteins observed during stress and recovery. Desiccation resulted in a decline in the abundance of proteins associated with photosynthesis and carbohydrate reserves, along with an increase in the presence of proteins associated with stress and defense response, such as peroxiredoxins and antioxidant enzymes. Following recovery, the abundance of stress-responsive proteins returned either partially or completely to their baseline level, confirming their importance to the seedling's desiccation response. Furthermore, proteins involved in carbohydrate metabolism, as well as fructose-bisphosphate aldolase and fructokinase-2 and phosphorylated metabolites as the substrate or the end-product, showed the inverse pattern during desiccation and after re-watering. This may reflect the fact that plants maintained energy supply during stress to protect seedlings from further damage, and for use in subsequent recovery after rewatering period. This study provides novel insights into the molecular mechanisms underlying the desiccation tolerance of wheat seedlings, and paves the way for more detailed molecular analysis of this remarkable phenomenon.

Original languageEnglish
Pages (from-to)349-362
Number of pages14
JournalPlant Physiology and Biochemistry
Volume146
DOIs
Publication statusPublished - Jan 2020

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Keywords

  • Desiccation tolerance
  • Drought stress
  • Proteomics
  • Recovery
  • Wheat
  • Triticum
  • Desiccation
  • Proteome
  • Plant Proteins
  • Seedlings

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