Proteomic characterization of the Chlamydomonas reinhardtii chloroplast ribosome

Identification of proteins unique to the 70 S ribosome

Kenichi Yamaguchi, María Verónica Beligni, Susana Prieto, Paul A. Haynes, W. Hayes McDonald, John R. Yates, Stephen P. Mayfield*

*Corresponding author for this work

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

We have conducted a proteomic analysis of the 70 S ribosome from the Chlamydomonas reinhardtii chloroplast. Twenty-seven orthologs of Escherichia coli large subunit proteins were identified in the 50 S subunit, as well as an ortholog of the spinach plastid-specific ribosomal protein-6. Several of the large subunit proteins of C. reinhardtii have short extension or insertion sequences, but overall the large subunit proteins are very similar to those of spinach chloroplast and E. coli. Two proteins of 38 and 41 kDa, designated RAP38 and RAP41, were identified from the 70 S ribosome that were not found in either of the ribosomal subunits. Phylogenetic analysis identified RAP38 and RAP41 as paralogs of spinach CSP41, a chloroplast RNA-binding protein with endoribonuclease activity. Overall, the chloroplast ribosome of C. reinhardtii is similar to those of spinach chloroplast and E. coli, but the C. reinhardtii ribosome has proteins associated with the 70 S complex that are related to non-ribosomal proteins in other species. In addition, the 30 S subunit contains unusually large orthologs of E. coli S2, S3, and S5 and a novel Sl-type protein (Yamaguchi, K. et al, (2002) Plant Cell 14, 2957-2974). These additional proteins and domains likely confer functions used to regulate chloroplast translation in C. reinhardtii.

Original languageEnglish
Pages (from-to)33774-33785
Number of pages12
JournalJournal of Biological Chemistry
Volume278
Issue number36
DOIs
Publication statusPublished - 5 Sep 2003
Externally publishedYes

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