Proteomic diversity in a prevalent human-infective Giardia duodenalis sub-species

Samantha J. Emery-Corbin*, Daniel Vuong, Ernest Lacey, Staffan G. Svärd, Brendan R. E. Ansell, Aaron R. Jex

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Giardia duodenalis a species complex of gastrointestinal protists, with assemblages A and B infective to humans. To date, post-genomic proteomics are largely derived from Assemblage A, biasing understanding of parasite biology. To address this gap, we quantitatively analysed the proteomes of trophozoites from the genome reference and two clinical Assemblage B isolates, revealing lower spectrum-to-peptide matches in non-reference isolates, resulting in significant losses in peptide and protein identifications, and indicating significant intra-assemblage variation. We also explored differential protein expression between in vitro cultured subpopulations putatively enriched for dividing and feeding cells, respectively. This data is an important proteomic baseline for Assemblage B, highlighting proteomic differences between physiological states, and unique differences relative to Assemblage A.

Original languageEnglish
Pages (from-to)817-823
Number of pages7
JournalInternational Journal for Parasitology
Volume48
Issue number11
DOIs
Publication statusPublished - Sep 2018

Keywords

  • Giardia duodenalis
  • Assemblage B
  • Label-free proteomics
  • Protozoology
  • Comparative proteomics

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