Proteomic response to linoleic acid hydroperoxide in Saccharomyces cerevisiae

Patrick J. O'Doherty, Alamgir Khan, Adam J. Johnson, Peter J. Rogers, Trevor D. Bailey, Ming J. Wu

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Yeast AP-1 transcription factor (Yap1p) and the enigmatic oxidoreductases Oye2p and Oye3p are involved in counteracting lipid oxidants and their unsaturated breakdown products. In order to uncover the response to linoleic acid hydroperoxide (LoaOOH) and the roles of Oye2p, Oye3p and Yap1p, we carried out proteomic analysis of the homozygous deletion mutants oye3Δ, oye2Δ and yap1Δ alongside the diploid parent strain BY4743. The findings demonstrate that deletion of YAP1 narrowed the response to LoaOOH, as the number of proteins differentially expressed in yap1Δ was 70% of that observed in BY4743. The role of Yap1p in regulating the major yeast peroxiredoxin Tsa1p was demonstrated by the decreased expression of Tsa1p in yap1Δ. The levels of Ahp1p and Hsp31p, previously shown to be regulated by Yap1p, were increased in LoaOOH-treated yap1Δ, indicating their expression is also regulated by another transcription factor(s). Relative to BY4743, protein expression differed in oye3Δ and oye2Δ under LoaOOH, underscored by superoxide dismutase (Sod1p), multiple heat shock proteins (Hsp60p, Ssa1p, and Sse1p), the flavodoxin-like protein Pst2p and the actin stabiliser tropomyosin (Tpm1p). Proteins associated with glycolysis were increased in all strains following treatment with LoaOOH. Together, the dataset reveals, for the first time, the yeast proteomic response to LoaOOH, highlighting the significance of carbohydrate metabolism, as well as distinction between the roles of Oye3p, Oye2p and Yap1p.

LanguageEnglish
Article numberfox022
Pages1-11
Number of pages11
JournalFEMS Yeast Research
Volume17
Issue number3
DOIs
Publication statusPublished - May 2017

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Proteomics
Saccharomyces cerevisiae
Yeasts
Proteins
Flavodoxin
Peroxiredoxins
Tropomyosin
Transcription Factor AP-1
Carbohydrate Metabolism
Glycolysis
Heat-Shock Proteins
Diploidy
Oxidants
Superoxide Dismutase
Actins
Oxidoreductases
Transcription Factors
Lipids
linoleic acid hydroperoxide

Keywords

  • Saccharomyces cerevisiae
  • proteomics
  • linoleic acid hydroperoxide
  • oxidative stress
  • old yellow enzyme
  • YAP1

Cite this

O'Doherty, P. J., Khan, A., Johnson, A. J., Rogers, P. J., Bailey, T. D., & Wu, M. J. (2017). Proteomic response to linoleic acid hydroperoxide in Saccharomyces cerevisiae. FEMS Yeast Research, 17(3), 1-11. [fox022]. https://doi.org/10.1093/femsyr/fox022
O'Doherty, Patrick J. ; Khan, Alamgir ; Johnson, Adam J. ; Rogers, Peter J. ; Bailey, Trevor D. ; Wu, Ming J. / Proteomic response to linoleic acid hydroperoxide in Saccharomyces cerevisiae. In: FEMS Yeast Research. 2017 ; Vol. 17, No. 3. pp. 1-11.
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O'Doherty, PJ, Khan, A, Johnson, AJ, Rogers, PJ, Bailey, TD & Wu, MJ 2017, 'Proteomic response to linoleic acid hydroperoxide in Saccharomyces cerevisiae', FEMS Yeast Research, vol. 17, no. 3, fox022, pp. 1-11. https://doi.org/10.1093/femsyr/fox022

Proteomic response to linoleic acid hydroperoxide in Saccharomyces cerevisiae. / O'Doherty, Patrick J.; Khan, Alamgir; Johnson, Adam J.; Rogers, Peter J.; Bailey, Trevor D.; Wu, Ming J.

In: FEMS Yeast Research, Vol. 17, No. 3, fox022, 05.2017, p. 1-11.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Proteomic response to linoleic acid hydroperoxide in Saccharomyces cerevisiae

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AU - Khan, Alamgir

AU - Johnson, Adam J.

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AU - Wu, Ming J.

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