Proteomic responses to drought vary widely among eight diverse genotypes of rice (Oryza sativa)

Sara Hamzelou; Dana Pascovici; Karthik Shantharam Kamath; Ardeshir Amirkhani; Matthew McKay; Mehdi Mirzaei; Brian J. Atwell; Paul A. Haynes

doi:10.3390/ijms21010363

Proteomic responses to drought vary widely among eight diverse genotypes of rice (Oryza sativa)

Sara Hamzelou, Dana Pascovici, Karthik Shantharam Kamath, Ardeshir Amirkhani, Matthew McKay, Mehdi Mirzaei, Brian J. Atwell, Paul A. Haynes^*

^*Corresponding author for this work

Australian Proteome Analysis Facility (APAF)

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

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Abstract

Rice is a critically important food source but yields worldwide are vulnerable to periods of drought. We exposed eight genotypes of upland and lowland rice (Oryza sativa L. ssp. japonica and indica) to drought stress at the late vegetative stage, and harvested leaves for label-free shotgun proteomics. Gene ontology analysis was used to identify common drought-responsive proteins in vegetative tissues, and leaf proteins that are unique to individual genotypes, suggesting diversity in the metabolic responses to drought. Eight proteins were found to be induced in response to drought stress in all eight genotypes. A total of 213 proteins were identified in a single genotype, 83 of which were increased in abundance in response to drought stress. In total, 10 of these 83 proteins were of a largely uncharacterized function, making them candidates for functional analysis and potential biomarkers for drought tolerance.

Original language	English
Article number	363
Pages (from-to)	1-16
Number of pages	16
Journal	International Journal of Molecular Sciences
Volume	21
Issue number	1
DOIs	https://doi.org/10.3390/ijms21010363
Publication status	Published - Jan 2020

Bibliographical note

Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

Drought stress
Rice
Shotgun proteomics
Label-free quantitation
Mass spectrometry

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10.3390/ijms21010363

Publisher version (open access)Final published version, 2.86 MBLicence: CC BY

Cite this

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title = "Proteomic responses to drought vary widely among eight diverse genotypes of rice (Oryza sativa)",

abstract = "Rice is a critically important food source but yields worldwide are vulnerable to periods of drought. We exposed eight genotypes of upland and lowland rice (Oryza sativa L. ssp. japonica and indica) to drought stress at the late vegetative stage, and harvested leaves for label-free shotgun proteomics. Gene ontology analysis was used to identify common drought-responsive proteins in vegetative tissues, and leaf proteins that are unique to individual genotypes, suggesting diversity in the metabolic responses to drought. Eight proteins were found to be induced in response to drought stress in all eight genotypes. A total of 213 proteins were identified in a single genotype, 83 of which were increased in abundance in response to drought stress. In total, 10 of these 83 proteins were of a largely uncharacterized function, making them candidates for functional analysis and potential biomarkers for drought tolerance.",

keywords = "Drought stress, Rice, Shotgun proteomics, Label-free quantitation, Mass spectrometry",

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note = "Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. ",

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AU - Hamzelou, Sara

AU - Pascovici, Dana

AU - Kamath, Karthik Shantharam

AU - Amirkhani, Ardeshir

AU - McKay, Matthew

AU - Mirzaei, Mehdi

AU - Atwell, Brian J.

AU - Haynes, Paul A.

N1 - Copyright the Author(s) 2020. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2020/1

Y1 - 2020/1

N2 - Rice is a critically important food source but yields worldwide are vulnerable to periods of drought. We exposed eight genotypes of upland and lowland rice (Oryza sativa L. ssp. japonica and indica) to drought stress at the late vegetative stage, and harvested leaves for label-free shotgun proteomics. Gene ontology analysis was used to identify common drought-responsive proteins in vegetative tissues, and leaf proteins that are unique to individual genotypes, suggesting diversity in the metabolic responses to drought. Eight proteins were found to be induced in response to drought stress in all eight genotypes. A total of 213 proteins were identified in a single genotype, 83 of which were increased in abundance in response to drought stress. In total, 10 of these 83 proteins were of a largely uncharacterized function, making them candidates for functional analysis and potential biomarkers for drought tolerance.

AB - Rice is a critically important food source but yields worldwide are vulnerable to periods of drought. We exposed eight genotypes of upland and lowland rice (Oryza sativa L. ssp. japonica and indica) to drought stress at the late vegetative stage, and harvested leaves for label-free shotgun proteomics. Gene ontology analysis was used to identify common drought-responsive proteins in vegetative tissues, and leaf proteins that are unique to individual genotypes, suggesting diversity in the metabolic responses to drought. Eight proteins were found to be induced in response to drought stress in all eight genotypes. A total of 213 proteins were identified in a single genotype, 83 of which were increased in abundance in response to drought stress. In total, 10 of these 83 proteins were of a largely uncharacterized function, making them candidates for functional analysis and potential biomarkers for drought tolerance.

KW - Drought stress

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KW - Shotgun proteomics

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KW - Mass spectrometry

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JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

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ER -

Proteomic responses to drought vary widely among eight diverse genotypes of rice (Oryza sativa)

Abstract

Bibliographical note

Keywords

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Re-engineering rice root architecture to maximise water use efficiency

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Proteomic responses to drought vary widely among eight diverse genotypes of rice (Oryza sativa)

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Projects

Re-engineering rice root architecture to maximise water use efficiency

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