Proteomic signature of human embryonic stem cells

Hossein Baharvand*, Mohsen Hajheidari, Saeid Kazemi Ashtiani, Ghasem Hosseini Salekdeh

*Corresponding author for this work

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Human embryonic stem cells (hESC) represent a population of undifferentiated pluripotent cells with both self-renewal and multilineage differentiation characteristics. Proteomics provides a powerful approach for studying the characteristics of hESC and discovering molecular markers. We have analyzed proteome profiles of three hESC lines using 2-DE and MALDITOF-TOF. Out of 844 spots analyzed with MALDITOF-TOF, 685 proteins were identified of which 60 proteins were classified as the most abundant proteins on 2-D gels. A large number of proteins particularly high abundant ones were identified as chaperones, heat shock proteins, ubiquitin/proteasome, and oxidative stress responsive proteins underscoring the ability of these cells to resist oxidative stress and increase the life span. Several proteins involved in cell proliferation and differentiation were also among the highly expressed proteins. Although overall expression pattern of three hESC were similar, 54 spots changed quantitatively and 14 spots changed qualitatively among the hESC cell lines. Most of these proteins were identified as proteins involved in cell growth, metabolism and signal transduction, which may affect the self-renewal and pluripotency. To our knowledge, this study represents the first proteomic dataset for hESC and provides a better insight into the biology of hESC. Proteome maps of hESC are accessible at http://www.RoyanProteomics.ir.

Original languageEnglish
Pages (from-to)3544-3549
Number of pages6
JournalProteomics
Volume6
Issue number12
DOIs
Publication statusPublished - 1 Jun 2006
Externally publishedYes

Keywords

  • Human embryonic stem cell
  • Proteome map
  • Two-dimensional gel electrophoresis

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