Proteomics and immunomapping of reactive lymph-node and lymphoma

Francesca Antonucci, Marco Chilosi, Maria Santacatterina, Ben Herbert, Pier Giorgio Righetti*

*Corresponding author for this work

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In the present study we show that two-dimensional (2-D) maps together with immuno-detection allow the precise identification of important leukocyte differentiation and tumor markers (e.g., CD3 and CD5), and important cell cycle regulatory molecules such as cyclin dependent kinases, notably CDK6. In addition, the comparative evaluation of molecular expression (e.g., CD5) in maps developed with normal and lymphoma samples can provide reproducible and precise information regarding the molecular expression in different cell populations. Accordingly, we could detect a much increased level of expression of CD5 in mantle cell lymphoma, up to ten times higher than in the control. In addition, CD5 in tumor tissues seems to be microheterogeneous as compared to normal samples.

Original languageEnglish
Pages (from-to)356-362
Number of pages7
JournalElectrophoresis
Volume23
Issue number2
DOIs
Publication statusPublished - 2002

Keywords

  • Immunoblotting
  • Mantle cell lymphoma
  • Two-dimensional maps

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    Antonucci, F., Chilosi, M., Santacatterina, M., Herbert, B., & Righetti, P. G. (2002). Proteomics and immunomapping of reactive lymph-node and lymphoma. Electrophoresis, 23(2), 356-362. https://doi.org/10.1002/1522-2683(200202)23:2<356::AID-ELPS356>3.0.CO;2-1