TY - JOUR
T1 - Proteomics and immunomapping of reactive lymph-node and lymphoma
AU - Antonucci, Francesca
AU - Chilosi, Marco
AU - Santacatterina, Maria
AU - Herbert, Ben
AU - Righetti, Pier Giorgio
PY - 2002
Y1 - 2002
N2 - In the present study we show that two-dimensional (2-D) maps together with immuno-detection allow the precise identification of important leukocyte differentiation and tumor markers (e.g., CD3 and CD5), and important cell cycle regulatory molecules such as cyclin dependent kinases, notably CDK6. In addition, the comparative evaluation of molecular expression (e.g., CD5) in maps developed with normal and lymphoma samples can provide reproducible and precise information regarding the molecular expression in different cell populations. Accordingly, we could detect a much increased level of expression of CD5 in mantle cell lymphoma, up to ten times higher than in the control. In addition, CD5 in tumor tissues seems to be microheterogeneous as compared to normal samples.
AB - In the present study we show that two-dimensional (2-D) maps together with immuno-detection allow the precise identification of important leukocyte differentiation and tumor markers (e.g., CD3 and CD5), and important cell cycle regulatory molecules such as cyclin dependent kinases, notably CDK6. In addition, the comparative evaluation of molecular expression (e.g., CD5) in maps developed with normal and lymphoma samples can provide reproducible and precise information regarding the molecular expression in different cell populations. Accordingly, we could detect a much increased level of expression of CD5 in mantle cell lymphoma, up to ten times higher than in the control. In addition, CD5 in tumor tissues seems to be microheterogeneous as compared to normal samples.
KW - Immunoblotting
KW - Mantle cell lymphoma
KW - Two-dimensional maps
UR - http://www.scopus.com/inward/record.url?scp=0036178234&partnerID=8YFLogxK
U2 - 10.1002/1522-2683(200202)23:2<356::AID-ELPS356>3.0.CO;2-1
DO - 10.1002/1522-2683(200202)23:2<356::AID-ELPS356>3.0.CO;2-1
M3 - Article
C2 - 11840544
AN - SCOPUS:0036178234
SN - 0173-0835
VL - 23
SP - 356
EP - 362
JO - Electrophoresis
JF - Electrophoresis
IS - 2
ER -