Proteomics dataset from 26th Dynasty Egyptian mummified remains sampled using minimally invasive skin sampling tape strips

Dylan Multari; Prathiba Ravishankar; Geraldine J. Sullivan; Ronika K. Power; Constance Lord; James A. Fraser; Paul A. Haynes

doi:10.1016/j.dib.2022.108562

Proteomics dataset from 26th Dynasty Egyptian mummified remains sampled using minimally invasive skin sampling tape strips

Dylan Multari, Prathiba Ravishankar, Geraldine J. Sullivan, Ronika K. Power, Constance Lord, James A. Fraser, Paul A. Haynes^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

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Abstract

Paleoproteomics typically involves the destructive sampling of precious bioarchaeological materials. This analysis aims to investigate the proteins identifiable via nanoLC-MS/MS from highly degraded 26th Dynasty Egyptian mummified human remains (NMR.29.1-8) after non-destructive sampling with commercially available dermatology-grade skin sampling tape strips. A collection of cranial and other bone fragments were sampled with the tape strips then subsequently analysed using a shotgun proteomics approach. The number of proteins identified using this method ranged from 18 to 437 at a peptide FDR of <1%. Deamidation ratios were assessed using an in-house R script, with asparagine deamidation averaging ∼20–30% and glutamine deamidation averaging ∼15–25%.

Original language	English
Article number	108562
Pages (from-to)	1-7
Number of pages	7
Journal	Data in Brief
Volume	45
Early online date	31 Aug 2022
DOIs	https://doi.org/10.1016/j.dib.2022.108562
Publication status	Published - Dec 2022

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Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

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title = "Proteomics dataset from 26th Dynasty Egyptian mummified remains sampled using minimally invasive skin sampling tape strips",

abstract = "Paleoproteomics typically involves the destructive sampling of precious bioarchaeological materials. This analysis aims to investigate the proteins identifiable via nanoLC-MS/MS from highly degraded 26th Dynasty Egyptian mummified human remains (NMR.29.1-8) after non-destructive sampling with commercially available dermatology-grade skin sampling tape strips. A collection of cranial and other bone fragments were sampled with the tape strips then subsequently analysed using a shotgun proteomics approach. The number of proteins identified using this method ranged from 18 to 437 at a peptide FDR of <1%. Deamidation ratios were assessed using an in-house R script, with asparagine deamidation averaging ∼20–30% and glutamine deamidation averaging ∼15–25%.",

keywords = "Paleoproteomics, Deamidation, Bioarchaeology, Cultural Heritage, mass spectrometry, Cultural heritage, Mass spectrometry",

author = "Dylan Multari and Prathiba Ravishankar and Sullivan, {Geraldine J.} and Power, {Ronika K.} and Constance Lord and Fraser, {James A.} and Haynes, {Paul A.}",

note = "Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher. ",

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AU - Multari, Dylan

AU - Ravishankar, Prathiba

AU - Sullivan, Geraldine J.

AU - Power, Ronika K.

AU - Lord, Constance

AU - Fraser, James A.

AU - Haynes, Paul A.

N1 - Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2022/12

Y1 - 2022/12

N2 - Paleoproteomics typically involves the destructive sampling of precious bioarchaeological materials. This analysis aims to investigate the proteins identifiable via nanoLC-MS/MS from highly degraded 26th Dynasty Egyptian mummified human remains (NMR.29.1-8) after non-destructive sampling with commercially available dermatology-grade skin sampling tape strips. A collection of cranial and other bone fragments were sampled with the tape strips then subsequently analysed using a shotgun proteomics approach. The number of proteins identified using this method ranged from 18 to 437 at a peptide FDR of <1%. Deamidation ratios were assessed using an in-house R script, with asparagine deamidation averaging ∼20–30% and glutamine deamidation averaging ∼15–25%.

AB - Paleoproteomics typically involves the destructive sampling of precious bioarchaeological materials. This analysis aims to investigate the proteins identifiable via nanoLC-MS/MS from highly degraded 26th Dynasty Egyptian mummified human remains (NMR.29.1-8) after non-destructive sampling with commercially available dermatology-grade skin sampling tape strips. A collection of cranial and other bone fragments were sampled with the tape strips then subsequently analysed using a shotgun proteomics approach. The number of proteins identified using this method ranged from 18 to 437 at a peptide FDR of <1%. Deamidation ratios were assessed using an in-house R script, with asparagine deamidation averaging ∼20–30% and glutamine deamidation averaging ∼15–25%.

KW - Paleoproteomics

KW - Deamidation

KW - Bioarchaeology

KW - Cultural Heritage

KW - mass spectrometry

KW - Cultural heritage

KW - Mass spectrometry

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SN - 2352-3409

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JO - Data in Brief

JF - Data in Brief

M1 - 108562

ER -

Proteomics dataset from 26th Dynasty Egyptian mummified remains sampled using minimally invasive skin sampling tape strips

Abstract

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