Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy

Margaret Simonian; Dyna Shirasaki; Vivienne S. Lee; David Bervini; Michael Grace; Rachel R. Ogorzalek Loo; Joseph A. Loo; Mark P. Molloy; Marcus A. Stoodley

doi:10.1186/s12014-018-9217-x

Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy

Margaret Simonian^*, Dyna Shirasaki, Vivienne S. Lee, David Bervini, Michael Grace, Rachel R. Ogorzalek Loo, Joseph A. Loo, Mark P. Molloy, Marcus A. Stoodley

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

24 Downloads (Pure)

Abstract

Background: Rapid identification of novel targets and advancement of a vascular targeting strategy requires a comprehensive assessment of AVM endothelial membrane protein changes in response to irradiation. The aim of this study is to provide additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery.

Methods: We employed in vivo biotinylation methodology that we developed, to label membrane proteins in the rat model of AVM post radiosurgery. Mass spectrometry expression (MS^E) analysis was used to identify and quantify surface protein expression between irradiated and non irradiated rats, which mimics a radiosurgical treatment approach.

Results: Our proteomics data revealed differentially expressed membrane proteins between irradiated and non irradiated rats, e.g. profilin-1, ESM-1, ion channel proteins, annexin A2 and lumican.

Conclusion: This work provides additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery.

Original language	English
Article number	43
Pages (from-to)	1-8
Number of pages	8
Journal	Clinical Proteomics
Volume	15
DOIs	https://doi.org/10.1186/s12014-018-9217-x
Publication status	Published - 26 Dec 2018

Bibliographical note

Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Keywords

In vivo biotinylation
AVM animal model
Membrane proteins
Radiosurgery

Access to Document

10.1186/s12014-018-9217-xLicence: CC BY

Publisher version (open access)Final published version, 753 KBLicence: CC BY

Cite this

Simonian, M., Shirasaki, D., Lee, V. S., Bervini, D., Grace, M., Loo, R. R. O., Loo, J. A., Molloy, M. P., & Stoodley, M. A. (2018). Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy. Clinical Proteomics, 15, 1-8. [43]. https://doi.org/10.1186/s12014-018-9217-x

@article{af010616308b4c819148969a202f9393,

title = "Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy",

abstract = "Background: Rapid identification of novel targets and advancement of a vascular targeting strategy requires a comprehensive assessment of AVM endothelial membrane protein changes in response to irradiation. The aim of this study is to provide additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery. Methods: We employed in vivo biotinylation methodology that we developed, to label membrane proteins in the rat model of AVM post radiosurgery. Mass spectrometry expression (MSE) analysis was used to identify and quantify surface protein expression between irradiated and non irradiated rats, which mimics a radiosurgical treatment approach. Results: Our proteomics data revealed differentially expressed membrane proteins between irradiated and non irradiated rats, e.g. profilin-1, ESM-1, ion channel proteins, annexin A2 and lumican. Conclusion: This work provides additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery.",

keywords = "In vivo biotinylation, AVM animal model, Membrane proteins, Radiosurgery",

author = "Margaret Simonian and Dyna Shirasaki and Lee, {Vivienne S.} and David Bervini and Michael Grace and Loo, {Rachel R. Ogorzalek} and Loo, {Joseph A.} and Molloy, {Mark P.} and Stoodley, {Marcus A.}",

note = "Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.",

year = "2018",

month = dec,

day = "26",

doi = "10.1186/s12014-018-9217-x",

language = "English",

volume = "15",

pages = "1--8",

journal = "Clinical Proteomics",

issn = "1542-6416",

publisher = "Springer, Springer Nature",

}

Simonian, M, Shirasaki, D, Lee, VS, Bervini, D, Grace, M, Loo, RRO, Loo, JA, Molloy, MP & Stoodley, MA 2018, 'Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy', Clinical Proteomics, vol. 15, 43, pp. 1-8. https://doi.org/10.1186/s12014-018-9217-x

TY - JOUR

T1 - Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy

AU - Simonian, Margaret

AU - Shirasaki, Dyna

AU - Lee, Vivienne S.

AU - Bervini, David

AU - Grace, Michael

AU - Loo, Rachel R. Ogorzalek

AU - Loo, Joseph A.

AU - Molloy, Mark P.

AU - Stoodley, Marcus A.

N1 - Copyright the Author(s) 2018. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

PY - 2018/12/26

Y1 - 2018/12/26

N2 - Background: Rapid identification of novel targets and advancement of a vascular targeting strategy requires a comprehensive assessment of AVM endothelial membrane protein changes in response to irradiation. The aim of this study is to provide additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery. Methods: We employed in vivo biotinylation methodology that we developed, to label membrane proteins in the rat model of AVM post radiosurgery. Mass spectrometry expression (MSE) analysis was used to identify and quantify surface protein expression between irradiated and non irradiated rats, which mimics a radiosurgical treatment approach. Results: Our proteomics data revealed differentially expressed membrane proteins between irradiated and non irradiated rats, e.g. profilin-1, ESM-1, ion channel proteins, annexin A2 and lumican. Conclusion: This work provides additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery.

AB - Background: Rapid identification of novel targets and advancement of a vascular targeting strategy requires a comprehensive assessment of AVM endothelial membrane protein changes in response to irradiation. The aim of this study is to provide additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery. Methods: We employed in vivo biotinylation methodology that we developed, to label membrane proteins in the rat model of AVM post radiosurgery. Mass spectrometry expression (MSE) analysis was used to identify and quantify surface protein expression between irradiated and non irradiated rats, which mimics a radiosurgical treatment approach. Results: Our proteomics data revealed differentially expressed membrane proteins between irradiated and non irradiated rats, e.g. profilin-1, ESM-1, ion channel proteins, annexin A2 and lumican. Conclusion: This work provides additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery.

KW - In vivo biotinylation

KW - AVM animal model

KW - Membrane proteins

KW - Radiosurgery

UR - http://www.scopus.com/inward/record.url?scp=85059096320&partnerID=8YFLogxK

U2 - 10.1186/s12014-018-9217-x

DO - 10.1186/s12014-018-9217-x

M3 - Article

C2 - 30602943

AN - SCOPUS:85059096320

SN - 1542-6416

VL - 15

SP - 1

EP - 8

JO - Clinical Proteomics

JF - Clinical Proteomics

M1 - 43

ER -

Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy

Abstract

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Cite this