Proteomics uncovers a role for redox in drought tolerance in wheat

Mohsen Hajheidari, Alireza Eivazi, Bob B. Buchanan, Joshua H. Wong, Islam Majidi, Ghasem Hosseini Salekdeh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

176 Citations (Scopus)


Proteomic analysis offers a new approach to identify a broad spectrum of genes that are expressed in living systems. We applied a proteomic approach to study changes in wheat grain in response to drought, a major environmental parameter adversely affecting development and crop yield. Three wheat genotypes differing in genetic background were cultivated in field under well-watered and drought conditions by following a randomized complete block design with four replications. The overall effect of drought was highly significant as determined by grain yield and total dry matter. About 650 spots were reproducibly detected and analyzed on 2-DE gels. Of these, 121 proteins showed significant change under drought condition in at least one of the genotypes. Mass spectrometry analysis using MALDI-TOF/TOF led to the identification of 57 proteins. Two-thirds of identified proteins were thioredoxin (Trx) targets, in accordance with the link between drought and oxidative stress. Further, because of contrasting changes in the tolerant and susceptible genotypes studied, several proteins emerge as key participants in the drought response. In addition to providing new information on the response to water deprivation, the present study offers opportunities to pursue the breeding of wheat with enhanced drought tolerance using identified candidate genetic markers. The 2-DE database of wheat seed proteins is available for public access at

Original languageEnglish
Pages (from-to)1451-1460
Number of pages10
JournalJournal of Proteome Research
Issue number4
Publication statusPublished - 1 Apr 2007
Externally publishedYes


  • Drought
  • Drought tolerance
  • Oxidative stress
  • Proteomics
  • Redox proteins
  • Seed
  • Thioredoxin h
  • Wheat


Dive into the research topics of 'Proteomics uncovers a role for redox in drought tolerance in wheat'. Together they form a unique fingerprint.

Cite this