Pulsed-field gel electrophoresis of genomic digests of Thermus strains and its implications for taxonomic and evolutionary studies

A. G. Rodrigo, K. M. Borges, P. L. Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Pulsed-field electrophoresis (PFGE) of the SspI genomic digests of 14 Thermus isolates showed that each one had a unique restriction enzyme digestion pattern. A group of New Zealand strains showed some shared bands, but each isolate gave essentially a unique fingerprint. In addition, evolutionary distances between Thermus strains estimated by using PFGE restriction fragment length polymorphisms (PFGE-RFLPs) correlate well with those based on small-subunit rRNA sequence data. As a consequence, the phylogenetic trees constructed on the basis of PFGE-RFLPs and those constructed by using small-subunit rRNA sequences generally agree. On the basis of the evolutionary distances estimated by using PFGE-RFLPs, the estimated average genomic rate of divergence for Thermus spp. is approximately 0.27% per million years.

Original languageEnglish
Pages (from-to)547-552
Number of pages6
JournalInternational Journal of Systematic Bacteriology
Volume44
Issue number3
Publication statusPublished - 1994
Externally publishedYes

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