Pulsed-field gel electrophoresis of pseudomonas aeruginosa

Honghua Hu, Jim Manos

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Pulsed-field gel electrophoresis (PFGE) is a technique used for the separation of high molecular weight restriction fragments from digested bacterial genomic DNA on a gel matrix by applying an electric field that periodically changes direction. PFGE has been shown to be the most discriminatory and reproducible bacterial strain typing technique available. It is considered the ‘gold standard’ in epidemiological studies of pathogenic and nonpathogenic microorganisms. This chapter provides a detailed method for using PFGE in the genotyping of the opportunistic Gram-negative bacterial pathogen Pseudomonas aeruginosa.

    Original languageEnglish
    Pages (from-to)157-170
    Number of pages14
    JournalMethods in Molecular Biology
    Volume1301
    DOIs
    Publication statusPublished - 2015

    Keywords

    • genotyping
    • pseudomonas aeruginosa
    • pulsed-field gel electrophoresis

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