Pulsed-field gel electrophoresis study of the genome of Caldocellum saccharolyticum

Kim M. Borges; Peter L. Bergquist

doi:10.1007/BF01576828

Pulsed-field gel electrophoresis study of the genome of Caldocellum saccharolyticum

Kim M. Borges, Peter L. Bergquist^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The genome of the anaerobic thermophilic eubacterium Caldocellum saccharolyticum was analyzed by pulsed field gel electrophoresis. The restriction endonucleases NheI and SstII cleaved the genome into 23 and 21 fragments, respectively, and the sums of the fragments gave a genome size of approximately 2.78 Mbp. Hybridization of probes for several cellulolytic and hemicellulolytic genes, as well as a 16S ribosomal RNA gene, gave complex signals on PFGE-separated restriction fragments. Mapping of the genome by use of linking clone hybridizations had been initiated.

Original language	English
Pages (from-to)	15-19
Number of pages	5
Journal	Current Microbiology
Volume	27
Issue number	1
DOIs	https://doi.org/10.1007/BF01576828
Publication status	Published - Jul 1993
Externally published	Yes

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abstract = "The genome of the anaerobic thermophilic eubacterium Caldocellum saccharolyticum was analyzed by pulsed field gel electrophoresis. The restriction endonucleases NheI and SstII cleaved the genome into 23 and 21 fragments, respectively, and the sums of the fragments gave a genome size of approximately 2.78 Mbp. Hybridization of probes for several cellulolytic and hemicellulolytic genes, as well as a 16S ribosomal RNA gene, gave complex signals on PFGE-separated restriction fragments. Mapping of the genome by use of linking clone hybridizations had been initiated.",

author = "Borges, {Kim M.} and Bergquist, {Peter L.}",

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AB - The genome of the anaerobic thermophilic eubacterium Caldocellum saccharolyticum was analyzed by pulsed field gel electrophoresis. The restriction endonucleases NheI and SstII cleaved the genome into 23 and 21 fragments, respectively, and the sums of the fragments gave a genome size of approximately 2.78 Mbp. Hybridization of probes for several cellulolytic and hemicellulolytic genes, as well as a 16S ribosomal RNA gene, gave complex signals on PFGE-separated restriction fragments. Mapping of the genome by use of linking clone hybridizations had been initiated.

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JO - Current Microbiology

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ER -

Pulsed-field gel electrophoresis study of the genome of Caldocellum saccharolyticum

Abstract

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