Abstract
Flow cytometry comprising the steps of: producing a flow of cells of velocity (v), in single file, through an interaction region (12) of length (d), where (d) is defined by the height of a pulsed laser source (11) of height (d), which beam shines onto the flow of cells which beam defines the interaction region; pulsing the laser source at a repetition rate of (f) onto the flow of cells where the time between pulses (l/f) is approximately equal to the transit time (t) of a cell in the interaction region; collecting (15) and detecting (16) the light scattered by or fluoresced from a cell in the interaction region; analysing (17) the detected light to determine the presence or other characteristics of said cell.
| Original language | English |
|---|---|
| Patent number | AU1991087689 |
| IPC | G01N 15/14,G01N 21/64,G01N 21/85 |
| Priority date | 29/10/90 |
| Publication status | Submitted - 24 Sept 1992 |