Pulsed laser flow cytometry

James Austin Piper (Inventor), Joseph Alan Narai (Inventor), Donald James Ramsay (Inventor)

Research output: Patent

Abstract

Flow cytometry comprising the steps of: producing a flow of cells of velocity (v), in single file, through an interaction region (12) of length (d), where (d) is defined by the height of a pulsed laser source (11) of height (d), which beam shines onto the flow of cells which beam defines the interaction region; pulsing the laser source at a repetition rate of (f) onto the flow of cells where the time between pulses (l/f) is approximately equal to the transit time (t) of a cell in the interaction region; collecting (15) and detecting (16) the light scattered by or fluoresced from a cell in the interaction region; analysing (17) the detected light to determine the presence or other characteristics of said cell.

Original languageEnglish
Patent numberWO1992008120
IPCG01N 15/14 2006.1
Priority date29/10/90
Filing date29/10/91
Publication statusSubmitted - 14 May 1992

Bibliographical note

Designated States
AT, AU, BB, BG, BR, CA, CH, DE, DK, ES, FI, GB, HU, JP, KP, KR, LK, LU, MG, MN, MW, NO, PL, RO, SD, SE, SU, US
European Patent Office (AT, BE, CH, DE, DK, ES, FR, GB, GR, IT, LU, NL, SE)
African Intellectual Property Organization (BF, BJ, CF, CG, CI, CM, GA, GN, ML, MR, SN, TD, TG)

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