TY - JOUR
T1 - Purification and characterization of a humoral opsonin from the solitary urochordate Styela clava
AU - Kelly, Karen L.
AU - Cooper, Edwin L.
AU - Raftos, David A.
PY - 1992
Y1 - 1992
N2 - 1. 1. We have previously identified opsonic activity in the plasma of the solitary urochordate, Styela clava. 2. 2. Here, we report the purification and further characterization of the opsonic molecule. 3. 3. Two purification methods were employed. 4. 4. Gel filtration yielded one strongly opsonic fraction that contained a single, electrophoretically-resolved protein. 5. 5. Opsonic activity was dose-dependent and sensitive to tryptic digestion and heat denaturation. 6. 6. SDS-PAGE and calibrated gel filtration indicated the opsonic protein was a 17.5 kDa monomer while isoelectrofocusing indicated a single pI of 7.0. 7. 7. In an alternative procedure, a similar opsonic activity and protein were isolated by affinity purification using whole yeast cells.
AB - 1. 1. We have previously identified opsonic activity in the plasma of the solitary urochordate, Styela clava. 2. 2. Here, we report the purification and further characterization of the opsonic molecule. 3. 3. Two purification methods were employed. 4. 4. Gel filtration yielded one strongly opsonic fraction that contained a single, electrophoretically-resolved protein. 5. 5. Opsonic activity was dose-dependent and sensitive to tryptic digestion and heat denaturation. 6. 6. SDS-PAGE and calibrated gel filtration indicated the opsonic protein was a 17.5 kDa monomer while isoelectrofocusing indicated a single pI of 7.0. 7. 7. In an alternative procedure, a similar opsonic activity and protein were isolated by affinity purification using whole yeast cells.
UR - http://www.scopus.com/inward/record.url?scp=0026457236&partnerID=8YFLogxK
U2 - 10.1016/0305-0491(92)90401-C
DO - 10.1016/0305-0491(92)90401-C
M3 - Article
C2 - 1458848
AN - SCOPUS:0026457236
VL - 103
SP - 749
EP - 753
JO - Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology
JF - Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology
SN - 1096-4959
IS - 3
ER -