Purification, crystallization and preliminary X-ray analysis of a phycourobilin-containing phycoerythrin

Stephan Ritter, Roger G. Hiller, Pamela M. Wrench, Thomas Wacker, Wolfram Welte, Kay Diederichs*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    3 Citations (Scopus)

    Abstract

    Rhodophycean phycoerythrin (R-PE) was isolated from the red alga Griffithsia monilis and purified on Q-Sepharose. Untwinned crystals diffracting to 2.1Å on a rotating anode X-ray source were obtained by the hanging-drop vapour diffusion method, and their space group determined as R3. SDS gel-electrophoresis of dissolved crystals showed the α and β subunits of the protein (apparent molecular weight, about 19 kDa) and two different forms of the γ-subunit (apparent molecular weight, 28 and 30 kDa) being present in the crystals.

    Original languageEnglish
    Pages (from-to)69-74
    Number of pages6
    JournalProtein and peptide letters
    Volume4
    Issue number1
    Publication statusPublished - 1997

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