Quantification of cytosol and membrane proteins in rumen epithelium of sheep with low or high CH4 emission phenotype

J. J. Bond*, A. J. Donaldson, S. Woodgate, K. S. Kamath, M. J. McKay, D. Wheeler, D. Tucker, V. H. Oddy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
22 Downloads (Pure)

Abstract

Background: Ruminant livestock are a major contributor to Australian agricultural sector carbon emissions. Variation in methane (CH4) produced from enteric microbial fermentation of feed in the reticulo-rumen of sheep differs with different digestive functions. 

Method: We isolated rumen epithelium enzymatically to extract membrane and cytosol proteins from sheep with high (H) and low (L) CH4 emission. Protein abundance was quantified using SWATH-mass spectrometry. 

Results: The research found differences related to the metabolism of glucose, lactate and processes of cell defence against microbes in sheep from each phenotype. Enzymes in the methylglyoxal pathway, a side path of glycolysis, resulting in D-lactate production, differed in abundance. In the H CH4 rumen epithelium the enzyme hydroxyacylglutathione hydrolase (HAGH) was 2.56 fold higher in abundance, whereas in the L CH4 epithelium lactate dehydrogenase D (LDHD) was 1.93 fold higher. Malic enzyme 1 which converts D-lactate to pyruvate via the tricarboxylic cycle was 1.57 fold higher in the L CH4 phenotype. Other proteins that are known to regulate cell defence against microbes had differential abundance in the epithelium of each phenotype. 

Conclusion: Differences in the abundance of enzymes involved in the metabolism of glucose were associated with H and L CH4 phenotype sheep. Potentially this represents an opportunity to use protein markers in the rumen epithelium to select low CH4 emitting sheep.

Original languageEnglish
Article numbere0273184
Pages (from-to)1-17
Number of pages17
JournalPLoS ONE
Volume17
Issue number10
DOIs
Publication statusPublished - 18 Oct 2022

Bibliographical note

Copyright the Author(s) 2022. Version archived for private and non-commercial use with the permission of the author/s and according to publisher conditions. For further rights please contact the publisher.

Fingerprint

Dive into the research topics of 'Quantification of cytosol and membrane proteins in rumen epithelium of sheep with low or high CH4 emission phenotype'. Together they form a unique fingerprint.

Cite this