Quantification of proteins from proteomic analysis

Zainab Noor, Subash Adhikari, Shoba Ranganathan, Abidali Mohamedali

    Research output: Chapter in Book/Report/Conference proceedingEntry for encyclopedia/dictionary/reference book

    1 Citation (Scopus)


    After protein identification, the quantification of proteins is one of the fundamental applications of proteomics. Accurate quantification has the potential to yield insights of the biology of organisms and most especially the reaction to changes in the internal or external environment. Mass spectrometry and subsequent informatics has also played a significant role in revolutionising, allowing thousands of proteins to be quantified in a single experiment. This article briefly discusses the most commonly used protein quantification methodologies in proteomics and provides a worked case study of protein quantification analysis using freely available tools of publicly available Data Independent Acquisition mass spectrometry (DIA-MS) data.
    Original languageEnglish
    Title of host publicationEncyclopedia of bioinformatics and computational biology
    Subtitle of host publicationABC of Bioinformatics
    EditorsMario Cannataro, Bruno Gaeta, Mohammad Asif Khan
    Place of PublicationAmsterdam; Oxford; Cambridge
    Number of pages20
    ISBN (Electronic)9780128114322
    ISBN (Print)9780128114148
    Publication statusPublished - 2019

    Bibliographical note

    Editors-in-chief: Shoba Ranganathan, Kenta Nakai, Christian Schönbach, Michael Gribskov.


    • Bioinformatics
    • Protein quantification
    • Data-independent acquisition
    • Bioinformatics analysis
    • Expression analysis
    • Quantitative proteomics
    • Statistical analysis
    • Reaction monitoring
    • Mass spectrometry
    • Label-free quantification
    • Up/down regulation


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