Original language | English |
---|---|
Title of host publication | Encyclopedia of bioinformatics and computational biology |
Subtitle of host publication | ABC of Bioinformatics |
Editors | Mario Cannataro, Bruno Gaeta, Mohammad Asif Khan |
Place of Publication | Amsterdam; Oxford; Cambridge |
Publisher | Elsevier |
Pages | 871-890 |
Number of pages | 20 |
Volume | 3 |
ISBN (Electronic) | 9780128114322 |
ISBN (Print) | 9780128114148 |
DOIs | |
Publication status | Published - 2019 |
Abstract
After protein identification, the quantification of proteins is one of the fundamental applications of proteomics. Accurate quantification has the potential to yield insights of the biology of organisms and most especially the reaction to changes in the internal or external environment. Mass spectrometry and subsequent informatics has also played a significant role in revolutionising, allowing thousands of proteins to be quantified in a single experiment. This article briefly discusses the most commonly used protein quantification methodologies in proteomics and provides a worked case study of protein quantification analysis using freely available tools of publicly available Data Independent Acquisition mass spectrometry (DIA-MS) data.
Bibliographical note
Editors-in-chief: Shoba Ranganathan, Kenta Nakai, Christian Schönbach, Michael Gribskov.Keywords
- Bioinformatics
- Protein quantification
- Data-independent acquisition
- Bioinformatics analysis
- Expression analysis
- Quantitative proteomics
- Statistical analysis
- Reaction monitoring
- Mass spectrometry
- Label-free quantification
- Up/down regulation