TY - JOUR
T1 - Quantitation of ATP-binding cassette subfamily-A transporter gene expression in primary human brain cells
AU - Kim, Woojin S.
AU - Guillemin, Gilles J.
AU - Glaros, Elias N.
AU - Lim, Chai K.
AU - Garner, Brett
PY - 2006/6
Y1 - 2006/6
N2 - Five ATP-binding cassette (ABC) subfamily-A transporters (ABCA1, ABCA2, ABCA3, ABCA7 and ABCA8) are expressed in the brain. These transporters may regulate brain lipid transport; however, their relative expression level in isolated human brain cells is unknown. We developed real-time polymerase chain reaction assays to quantify the expression of these genes in human neurons, astrocytes, oligodendrocytes, microglia and cell lines. Neurons expressed predominantly ABCA1 and ABCA3; astrocytes ABCA1, ABCA2 and ABCA3; microglia ABCA1 and oligodendrocytes ABCA2 and ABCA3. Although ABCA7 and ABCA8 expression was relatively low in all cells, the highest expression occurred in microglia and neurons, respectively. ABCA gene expression in the NTERA-2 and MO3.13 cell lines closely resembled the ABCA expression pattern of primary neurons and oligodendrocytes, respectively.
AB - Five ATP-binding cassette (ABC) subfamily-A transporters (ABCA1, ABCA2, ABCA3, ABCA7 and ABCA8) are expressed in the brain. These transporters may regulate brain lipid transport; however, their relative expression level in isolated human brain cells is unknown. We developed real-time polymerase chain reaction assays to quantify the expression of these genes in human neurons, astrocytes, oligodendrocytes, microglia and cell lines. Neurons expressed predominantly ABCA1 and ABCA3; astrocytes ABCA1, ABCA2 and ABCA3; microglia ABCA1 and oligodendrocytes ABCA2 and ABCA3. Although ABCA7 and ABCA8 expression was relatively low in all cells, the highest expression occurred in microglia and neurons, respectively. ABCA gene expression in the NTERA-2 and MO3.13 cell lines closely resembled the ABCA expression pattern of primary neurons and oligodendrocytes, respectively.
UR - http://www.scopus.com/inward/record.url?scp=33745054649&partnerID=8YFLogxK
U2 - 10.1097/01.wnr.0000221833.41340.cd
DO - 10.1097/01.wnr.0000221833.41340.cd
M3 - Article
C2 - 16738483
AN - SCOPUS:33745054649
SN - 0959-4965
VL - 17
SP - 891
EP - 896
JO - NeuroReport
JF - NeuroReport
IS - 9
ER -