Quantitative age-specific variability of plasma proteins in healthy neonates, children and adults

Stefan Bjelosevic, Dana Pascovici, Hui Ping, Vasiliki Karlaftis, Thiri Zaw, Xiaomin Song, Mark P. Molloy, Paul Monagle, Vera Ignjatovic

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Human blood plasma is a complex biological fluid containing soluble proteins, sugars, hormones, electrolytes, and dissolved gasses. As plasma interacts with a wide array of bodily systems, changes in protein expression, or the presence or absence of specific proteins are regularly used in the clinic as a molecular biomarker tool. A large body of literature exists detailing proteomic changes in pathologic contexts, however little research has been conducted on the quantitation of the plasma proteome in age-specific, healthy subjects, especially in pediatrics. In this study, we utilized SWATH-MS to identify and quantify proteins in the blood plasma of healthy neonates, infants under 1 year of age, children between 1-5 years, and adults. We identified more than 100 proteins that showed significant differential expression levels across these age groups, and we analyzed variation in protein expression across the age spectrum. The plasma proteomic profiles of neonates were strikingly dissimilar to the older children and adults. By extracting the SWATH data against a large human spectral library we increased protein identification more than 6-fold (940 proteins) and confirmed the concentrations of several of these using ELISA. The results of this study map the variation in expression of proteins and pathways often implicated in disease, and so have significant clinical implication.

LanguageEnglish
Pages924-935
Number of pages12
JournalMolecular and Cellular Proteomics
Volume16
Issue number5
DOIs
Publication statusPublished - 1 May 2017

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Blood Proteins
Newborn Infant
Proteins
Plasmas
Proteomics
Blood
Pediatrics
Biomarkers
Proteome
Sugars
Electrolytes
Libraries
Healthy Volunteers
Age Groups
Enzyme-Linked Immunosorbent Assay
Hormones
Fluids
Research

Cite this

Bjelosevic, Stefan ; Pascovici, Dana ; Ping, Hui ; Karlaftis, Vasiliki ; Zaw, Thiri ; Song, Xiaomin ; Molloy, Mark P. ; Monagle, Paul ; Ignjatovic, Vera. / Quantitative age-specific variability of plasma proteins in healthy neonates, children and adults. In: Molecular and Cellular Proteomics. 2017 ; Vol. 16, No. 5. pp. 924-935.
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Quantitative age-specific variability of plasma proteins in healthy neonates, children and adults. / Bjelosevic, Stefan; Pascovici, Dana; Ping, Hui; Karlaftis, Vasiliki; Zaw, Thiri; Song, Xiaomin; Molloy, Mark P.; Monagle, Paul; Ignjatovic, Vera.

In: Molecular and Cellular Proteomics, Vol. 16, No. 5, 01.05.2017, p. 924-935.

Research output: Contribution to journalArticleResearchpeer-review

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