Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy

Annemarie Nadort, Rutger G. Woolthuis, Ton G. van Leeuwen, Dirk J. Faber

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)
54 Downloads (Pure)


We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, noninvasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDFLSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources.

Original languageEnglish
Pages (from-to)2347-2361
Number of pages15
JournalBiomedical Optics Express
Issue number11
Publication statusPublished - 1 Nov 2013

Bibliographical note

This paper was published in Biomedical optics express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/boe/abstract.cfm?uri=boe-4-11-2347. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.


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