Quantitative mass spectrometry for colorectal cancer proteomics

Juan Martínez-Aguilar, Jenny Chik, Judith Nicholson, Crystal Semaan, Matthew J. Mckay, Mark P. Molloy*

*Corresponding author for this work

Research output: Contribution to journalReview article

14 Citations (Scopus)

Abstract

This review documents the uses of quantitative MS applied to colorectal cancer (CRC) proteomics for biomarker discovery and molecular pathway profiling. Investigators are adopting various labeling and label-free MS approaches to quantitate differential protein levels in cells, tumors, and plasma/serum. We comprehensively review recent uses of this technology to examine mouse models of CRC, CRC cell lines, their secretomes and subcellular fractions, CRC tumors, CRC patient plasma/serum, and stool samples. For biomarker discovery these approaches are uncovering proteins with potential diagnostic and prognostic utility, while in vitro cell culture experiments are characterizing proteomic and phosphoproteomic responses to disrupted signaling pathways due to mutations or to inhibition of drugable enzymes.

Original languageEnglish
Pages (from-to)42-54
Number of pages13
JournalProteomics - Clinical Applications
Volume7
Issue number1-2
DOIs
Publication statusPublished - Jan 2013

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