Quantitative PCR for detection of mRNA and gDNA in environmental isolates

Anthony J. Brzoska; Karl A. Hassan

doi:10.1007/978-1-62703-712-9_3

Quantitative PCR for detection of mRNA and gDNA in environmental isolates

Anthony J. Brzoska, Karl A. Hassan

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

6 Citations (Scopus)

Abstract

Quantitative PCR is used to gauge the abundance of specific nucleic acid species within purified samples. Due to its high sensitivity and minimal operation costs, this method is routinely applied in modern molecular bioscience laboratories. Nonetheless, all quantitative PCR experiments must include several carefully designed, yet simple, controls to ensure the reliability of the analyses. The aim of this chapter is to provide basic quantitative PCR methods, from primer design through data analysis, that are generally applicable to studies in microbiology. These methods allow the abundance of targeted RNA or DNA molecules to be determined in nucleic acid samples purified from a variety of biological sources.

Original language	English
Title of host publication	Environmental Microbiology
Subtitle of host publication	Methods and Protocols
Editors	Ian T. Paulsen, Andrew J. Holmes
Place of Publication	Totowa, NJ
Publisher	Humana Press
Pages	25-42
Number of pages	18
Edition	2nd
ISBN (Electronic)	9781627037129
ISBN (Print)	9781627037112
DOIs	https://doi.org/10.1007/978-1-62703-712-9_3
Publication status	Published - 2014

Publication series

Name	Methods in Molecular Biology
Volume	1096
ISSN (Print)	1064-3745

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10.1007/978-1-62703-712-9_3

Cite this

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Quantitative PCR for detection of mRNA and gDNA in environmental isolates. / Brzoska, Anthony J.; Hassan, Karl A.
Environmental Microbiology: Methods and Protocols. ed. / Ian T. Paulsen; Andrew J. Holmes. 2nd. ed. Totowa, NJ: Humana Press, 2014. p. 25-42 (Methods in Molecular Biology; Vol. 1096).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

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AU - Hassan, Karl A.

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AB - Quantitative PCR is used to gauge the abundance of specific nucleic acid species within purified samples. Due to its high sensitivity and minimal operation costs, this method is routinely applied in modern molecular bioscience laboratories. Nonetheless, all quantitative PCR experiments must include several carefully designed, yet simple, controls to ensure the reliability of the analyses. The aim of this chapter is to provide basic quantitative PCR methods, from primer design through data analysis, that are generally applicable to studies in microbiology. These methods allow the abundance of targeted RNA or DNA molecules to be determined in nucleic acid samples purified from a variety of biological sources.

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Quantitative PCR for detection of mRNA and gDNA in environmental isolates

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Functional genomic analysis of multidrug efflux in the emerging pathogen Acinetobacter baumannii

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Quantitative PCR for detection of mRNA and gDNA in environmental isolates

Abstract

Publication series

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Functional genomic analysis of multidrug efflux in the emerging pathogen Acinetobacter baumannii

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