Projects per year
Quantitative PCR is used to gauge the abundance of specific nucleic acid species within purified samples. Due to its high sensitivity and minimal operation costs, this method is routinely applied in modern molecular bioscience laboratories. Nonetheless, all quantitative PCR experiments must include several carefully designed, yet simple, controls to ensure the reliability of the analyses. The aim of this chapter is to provide basic quantitative PCR methods, from primer design through data analysis, that are generally applicable to studies in microbiology. These methods allow the abundance of targeted RNA or DNA molecules to be determined in nucleic acid samples purified from a variety of biological sources.
|Title of host publication||Environmental Microbiology|
|Subtitle of host publication||Methods and Protocols|
|Editors||Ian T. Paulsen, Andrew J. Holmes|
|Place of Publication||Totowa, NJ|
|Number of pages||18|
|Publication status||Published - 2014|
|Name||Methods in Molecular Biology|
FingerprintDive into the research topics of 'Quantitative PCR for detection of mRNA and gDNA in environmental isolates'. Together they form a unique fingerprint.
- 1 Finished