Quantitative proteomics analysis of high and low polyphenol expressing recombinant inbred lines (RILs) of peanut (Arachis hypogaea L.)

Sridevi Muralidharan, Yan Yee Poon, Graeme C. Wright, Paul A. Haynes, Nanju A. Lee*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

To facilitate selective breeding of polyphenol-rich peanuts, we looked for mass spectrometry-based proteomic evidence, investigating a subset of recombinant inbred lines (RILs) developed by the Australian peanut breeding program. To do this, we used label-free shotgun proteomics for protein and peptide quantitation, statistically analyzed normalized spectral abundance factors using R-package, as well as assayed important antioxidants. Results revealed statistically significant protein expression changes in 82 proteins classified between high or low polyphenols expressing RILs. Metabolic changes in polyphenol-rich RIL p27-362 point towards increased enzymatic breakdown of sugars and phenylalanine biosynthesis. The study revealed phenylpropanoid pathway overexpression resulting in increased polyphenols biosynthesis. Overexpression of antioxidant enzymes such as catalase, by 73.4 fold was also observed. A strong metabolic correlation exists with the observed phenotypic traits. Peanut RIL p27-362 presents a superior nutritional composition with antioxidant-rich peanut phenotype and could yield commercial profits. Data are available via ProteomeXchange with identifier PXD015493.

Original languageEnglish
Article number127517
Number of pages13
JournalFood Chemistry
Volume334
DOIs
Publication statusPublished - 1 Jan 2021

Keywords

  • Label-free shotgun proteomics
  • LC-MS/MS
  • Phenylpropanoid pathway
  • Polyphenols
  • Protein biomarkers

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