Radical initiated α-tocopherol depletion and lipid peroxidation in mitochondrial membranes

Sian M. Thomas*, Janusz M. Gebicki, Roger T. Dean

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    62 Citations (Scopus)


    The relationships between antioxidant status, lipid peroxidation and membrane protein integrity have been studied in an isolated mitochondrial membrane system. Tocopherol was shown to be present in both the outer and inner membrane of normal rat liver mitochondria; 77.3 and 22.3% of the total α-tocopherol was present in the outer and inner membranes, respectively. The endogenous α-tocopherol was depleted in a time-dependent manner by low levels of ferrous iron and by irradiation in the presence or absence of ferrous iron. This antioxidant depletion was followed by the appearance of lipid hydroperoxides. Fragmentation of monoamine oxidase, an integral outer membrame protein, was observed at irradiation doses that caused both antioxidant depletion and peroxide generation.

    Original languageEnglish
    Pages (from-to)189-197
    Number of pages9
    JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
    Issue number2
    Publication statusPublished - 3 Apr 1989


    • (Rat)
    • Lipid peroxidation
    • Mitochondrial membrane
    • Protein fragmentation
    • Radical stress
    • Tocopherol depletion


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