Random drift mutagenesis

Peter Leonard Bergquist (Inventor), Moreland David Gibbs (Inventor), Rosalind Reeves (Inventor)

Research output: Patent

Abstract

Disclosed is a method for producing a mutant gene encoding a functional gene product comprising: (a) introducing one or more mutations into a gene to form a mutated gene; (b) providing the mutated gene to a host microorganism; (c) culturing the host microorganism containing the mutated gene under conditions which allow expression of the mutant gene product; (d) sorting the host bacteria by flow cytometry and (e) obtaining a combined pool of mutated genes encoding functional enzymes.

Original languageEnglish
Patent numberNZ539399
IPCC12N 15/ 10 A I,C12N 15/11
Priority date7/10/03
Publication statusPublished - 28 Nov 2008

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