Abstract
Disclosed is a method for producing a mutant gene encoding a functional gene product comprising: (a) introducing one or more mutations into a gene to form a mutated gene; (b) providing the mutated gene to a host microorganism; (c) culturing the host microorganism containing the mutated gene under conditions which allow expression of the mutant gene product; (d) sorting the host bacteria by flow cytometry and (e) obtaining a combined pool of mutated genes encoding functional enzymes.
| Original language | English |
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| Patent number | NZ539399 |
| IPC | C12N 15/11 |
| Priority date | 4/10/02 |
| Publication status | Published - 12 Mar 2009 |