Random drift mutagenesis

Peter Leonard Bergquist; Moreland David Gibbs; Rosalind Reeves

Random drift mutagenesis

Peter Leonard Bergquist (Inventor), Moreland David Gibbs (Inventor), Rosalind Reeves (Inventor)

Macquarie University

Research output: Patent

Abstract

Disclosed is a method for producing a mutant gene encoding a functional gene product comprising: (a) introducing one or more mutations into a gene to form a mutated gene; (b) providing the mutated gene to a host microorganism; (c) culturing the host microorganism containing the mutated gene under conditions which allow expression of the mutant gene product; (d) sorting the host bacteria by flow cytometry and (e) obtaining a combined pool of mutated genes encoding functional enzymes.

Original language	English
Patent number	NZ539399
IPC	C12N 15/11
Priority date	4/10/02
Publication status	Published - 12 Mar 2009

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https://patentscope.wipo.int/search/en/detail.jsf?docId=NZ179061305

2 Patent

Random drift mutagenesis
Bergquist, P. L., Gibbs, M. D. & Reeves, R., 29 Jun 2005, (Submitted) IPC No. C12N 15/10, Patent No. EP1546330, Priority date 4 Oct 2002, Priority No. AU2002951899
Research output: Patent
Random drift mutagenesis
Bergquist, P. L., Gibbs, M. D. & Reeves, R., 15 Apr 2004, (Submitted) IPC No. C12N 15/10 2006.1, Patent No. WO2004031387, Priority date 15 Apr 2004, Priority No. AU2002951899
Research output: Patent

Cite this

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title = "Random drift mutagenesis",

abstract = "Disclosed is a method for producing a mutant gene encoding a functional gene product comprising: (a) introducing one or more mutations into a gene to form a mutated gene; (b) providing the mutated gene to a host microorganism; (c) culturing the host microorganism containing the mutated gene under conditions which allow expression of the mutant gene product; (d) sorting the host bacteria by flow cytometry and (e) obtaining a combined pool of mutated genes encoding functional enzymes.",

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AU - Bergquist, Peter Leonard

AU - Gibbs, Moreland David

AU - Reeves, Rosalind

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N2 - Disclosed is a method for producing a mutant gene encoding a functional gene product comprising: (a) introducing one or more mutations into a gene to form a mutated gene; (b) providing the mutated gene to a host microorganism; (c) culturing the host microorganism containing the mutated gene under conditions which allow expression of the mutant gene product; (d) sorting the host bacteria by flow cytometry and (e) obtaining a combined pool of mutated genes encoding functional enzymes.

AB - Disclosed is a method for producing a mutant gene encoding a functional gene product comprising: (a) introducing one or more mutations into a gene to form a mutated gene; (b) providing the mutated gene to a host microorganism; (c) culturing the host microorganism containing the mutated gene under conditions which allow expression of the mutant gene product; (d) sorting the host bacteria by flow cytometry and (e) obtaining a combined pool of mutated genes encoding functional enzymes.

M3 - Patent

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Random drift mutagenesis

Abstract

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