Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis

Wai-Fong Chan; Ram P. Maharjan; Peter R. Reeves; Vitali Sintchenko; Gwendolyn L. Gilbert; Ruiting Lan

doi:10.1016/j.mimet.2009.03.007

Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis

Wai-Fong Chan, Ram P. Maharjan, Peter R. Reeves, Vitali Sintchenko, Gwendolyn L. Gilbert, Ruiting Lan

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Real Time-PCR (RT-PCR) and high resolution melt (HRM) analyses were used for rapid typing of genes encoding components of the pertussis acellular vaccine, namely prn, ptxA, fhaB, fim2 and fim3. The length polymorphisms in prn were detected by RT-PCR followed by HRM; single nucleotide polymorphisms in prn and other genes were detected by hairpin primer RT-PCR. These rapid methods are suitable for large-scale studies of vaccine-driven evolution of Bordetella pertussis.

Original language	English
Pages (from-to)	326-329
Number of pages	4
Journal	Journal of Microbiological Methods
Volume	77
Issue number	3
DOIs	https://doi.org/10.1016/j.mimet.2009.03.007
Publication status	Published - Jun 2009
Externally published	Yes

Keywords

Whooping cough
Single nucleotide polymorphism
Antigenic variation
Length polymorphism
High resolution melt analysis

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10.1016/j.mimet.2009.03.007

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title = "Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis",

abstract = "Real Time-PCR (RT-PCR) and high resolution melt (HRM) analyses were used for rapid typing of genes encoding components of the pertussis acellular vaccine, namely prn, ptxA, fhaB, fim2 and fim3. The length polymorphisms in prn were detected by RT-PCR followed by HRM; single nucleotide polymorphisms in prn and other genes were detected by hairpin primer RT-PCR. These rapid methods are suitable for large-scale studies of vaccine-driven evolution of Bordetella pertussis.",

keywords = "Whooping cough, Single nucleotide polymorphism, Antigenic variation, Length polymorphism, High resolution melt analysis",

author = "Wai-Fong Chan and Maharjan, \{Ram P.\} and Reeves, \{Peter R.\} and Vitali Sintchenko and Gilbert, \{Gwendolyn L.\} and Ruiting Lan",

year = "2009",

month = jun,

doi = "10.1016/j.mimet.2009.03.007",

language = "English",

volume = "77",

pages = "326--329",

journal = "Journal of Microbiological Methods",

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T1 - Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis

AU - Chan, Wai-Fong

AU - Maharjan, Ram P.

AU - Reeves, Peter R.

AU - Sintchenko, Vitali

AU - Gilbert, Gwendolyn L.

AU - Lan, Ruiting

PY - 2009/6

Y1 - 2009/6

N2 - Real Time-PCR (RT-PCR) and high resolution melt (HRM) analyses were used for rapid typing of genes encoding components of the pertussis acellular vaccine, namely prn, ptxA, fhaB, fim2 and fim3. The length polymorphisms in prn were detected by RT-PCR followed by HRM; single nucleotide polymorphisms in prn and other genes were detected by hairpin primer RT-PCR. These rapid methods are suitable for large-scale studies of vaccine-driven evolution of Bordetella pertussis.

AB - Real Time-PCR (RT-PCR) and high resolution melt (HRM) analyses were used for rapid typing of genes encoding components of the pertussis acellular vaccine, namely prn, ptxA, fhaB, fim2 and fim3. The length polymorphisms in prn were detected by RT-PCR followed by HRM; single nucleotide polymorphisms in prn and other genes were detected by hairpin primer RT-PCR. These rapid methods are suitable for large-scale studies of vaccine-driven evolution of Bordetella pertussis.

KW - Whooping cough

KW - Single nucleotide polymorphism

KW - Antigenic variation

KW - Length polymorphism

KW - High resolution melt analysis

UR - https://www.scopus.com/pages/publications/67349172946

U2 - 10.1016/j.mimet.2009.03.007

DO - 10.1016/j.mimet.2009.03.007

M3 - Article

C2 - 19341769

SN - 0167-7012

VL - 77

SP - 326

EP - 329

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 3

ER -

Rapid and accurate typing of Bordetella pertussis targeting genes encoding acellular vaccine antigens using real time PCR and High Resolution Melt analysis

Abstract

Keywords

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