Rapid and sensitive fusion gene detection in prostate cancer urinary specimens by label-free surface-enhanced Raman scattering

Kevin M. Koo, Benjamin McNamara, Eugene J. H. Wee, Yuling Wang*, Matt Trau

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Recurrent chromosomal rearrangements such as fusion genes are associated with cancer initiation and progression. Prostate cancer (PCa) is a leading cause of cancer-related deaths in men and the TMPRSS2-ERG gene fusion is a recurrent biomarker in about 50% of all prostate cancers. However, current screening tools for TMPRSS2-ERG are generally confined to research settings and hence, the development of a rapid, sensitive and accurate assay for TMPRSS2-ERG detection may aid in clinical PCa diagnosis and treatment. Herein, we described a new strategy for non-invasive TMPRSS2-ERG detection in patient urinary samples by coupling of isothermal reverse transcription-recombinase polymerization amplification (RT-RPA) to amplify TMPRSS2-ERG transcripts and surface-enhanced Raman scattering (SERS) to directly detect the amplicons. This novel coupling of both techniques allows rapid and quantitative TMPRSS2-ERG detection. Our assay can specifically detect as low as 103 copies input of TMPRSS2-ERG transcripts and was successfully applied to clinical PCa urinary samples. Hence, we believe our assay is a potential clinical screening tool for TMPRSS2-ERG in PCa and may have broad applications in detecting other gene fusion transcripts in other diseases.

Original languageEnglish
Pages (from-to)1798-1805
Number of pages8
JournalJournal of Biomedical Nanotechnology
Volume12
Issue number9
DOIs
Publication statusPublished - 1 Sep 2016
Externally publishedYes

Keywords

  • Fusion genes
  • Isothermal amplification
  • Label-free surface-enhanced Raman scattering
  • Prostate cancer
  • Urine samples

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