Rapid cloning of thermoalkalophilic lipases from Bacillus spp. using PCR

Philip J. L. Bell, Helena Nevalainen, Hugh W. Morgan, Peter L. Bergquist*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)


    A lipase gene from a thermophilic Bacillus sp. TG43, whose product showed optimal activity at alkaline pH, was cloned using a lambda expression library. Consensus PCR primers were designed based on a DNA sequence alignment of the cloned lipase with two other homologous lipases reported in the literature. The consensus primers allowed rapid cloning and expression of several novel lipases from DNA of both pure cultures of Bacillus and biomass from thermophilic environmental samples.

    Original languageEnglish
    Pages (from-to)1003-1006
    Number of pages4
    JournalBiotechnology Letters
    Issue number11
    Publication statusPublished - Nov 1999


    • bacillus
    • gene cloning
    • lipase
    • thermophile


    Dive into the research topics of 'Rapid cloning of thermoalkalophilic lipases from Bacillus spp. using PCR'. Together they form a unique fingerprint.

    Cite this