Rapid cloning of thermoalkalophilic lipases from Bacillus spp. using PCR

Philip J. L. Bell, Helena Nevalainen, Hugh W. Morgan, Peter L. Bergquist*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

A lipase gene from a thermophilic Bacillus sp. TG43, whose product showed optimal activity at alkaline pH, was cloned using a lambda expression library. Consensus PCR primers were designed based on a DNA sequence alignment of the cloned lipase with two other homologous lipases reported in the literature. The consensus primers allowed rapid cloning and expression of several novel lipases from DNA of both pure cultures of Bacillus and biomass from thermophilic environmental samples.

Original languageEnglish
Pages (from-to)1003-1006
Number of pages4
JournalBiotechnology Letters
Volume21
Issue number11
DOIs
Publication statusPublished - Nov 1999

Keywords

  • bacillus
  • gene cloning
  • lipase
  • thermophile

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