Rapid cloning of thermoalkalophilic lipases from Bacillus spp. using PCR

Philip J. L. Bell; Helena Nevalainen; Hugh W. Morgan; Peter L. Bergquist

doi:10.1023/A:1005654701905

Rapid cloning of thermoalkalophilic lipases from Bacillus spp. using PCR

Philip J. L. Bell, Helena Nevalainen, Hugh W. Morgan, Peter L. Bergquist^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

A lipase gene from a thermophilic Bacillus sp. TG43, whose product showed optimal activity at alkaline pH, was cloned using a lambda expression library. Consensus PCR primers were designed based on a DNA sequence alignment of the cloned lipase with two other homologous lipases reported in the literature. The consensus primers allowed rapid cloning and expression of several novel lipases from DNA of both pure cultures of Bacillus and biomass from thermophilic environmental samples.

Original language	English
Pages (from-to)	1003-1006
Number of pages	4
Journal	Biotechnology Letters
Volume	21
Issue number	11
DOIs	https://doi.org/10.1023/A:1005654701905
Publication status	Published - Nov 1999

Keywords

bacillus
gene cloning
lipase
thermophile

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10.1023/A:1005654701905

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abstract = "A lipase gene from a thermophilic Bacillus sp. TG43, whose product showed optimal activity at alkaline pH, was cloned using a lambda expression library. Consensus PCR primers were designed based on a DNA sequence alignment of the cloned lipase with two other homologous lipases reported in the literature. The consensus primers allowed rapid cloning and expression of several novel lipases from DNA of both pure cultures of Bacillus and biomass from thermophilic environmental samples.",

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AU - Bergquist, Peter L.

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AB - A lipase gene from a thermophilic Bacillus sp. TG43, whose product showed optimal activity at alkaline pH, was cloned using a lambda expression library. Consensus PCR primers were designed based on a DNA sequence alignment of the cloned lipase with two other homologous lipases reported in the literature. The consensus primers allowed rapid cloning and expression of several novel lipases from DNA of both pure cultures of Bacillus and biomass from thermophilic environmental samples.

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KW - thermophile

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Rapid cloning of thermoalkalophilic lipases from Bacillus spp. using PCR

Abstract

Keywords

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