A lipase gene from a thermophilic Bacillus sp. TG43, whose product showed optimal activity at alkaline pH, was cloned using a lambda expression library. Consensus PCR primers were designed based on a DNA sequence alignment of the cloned lipase with two other homologous lipases reported in the literature. The consensus primers allowed rapid cloning and expression of several novel lipases from DNA of both pure cultures of Bacillus and biomass from thermophilic environmental samples.
- gene cloning