Rapid detection of Paenibacillus larvae from honey and hive samples with a novel nested PCR protocol

Federico M. Lauro, Matteo Favaretto, Loredana Covolo, Mario Rassu, Giulio Bertoloni*

*Corresponding author for this work

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

The bacterial pathogen Paenibacillus larvae is the causative agent of American foulbrood disease in honeybees (Apis mellifera). A touchdown nested PCR protocol was developed to detect the presence of P. larvae spores directly in honey and hive samples. This approach allows early discovery of the bacteria even at concentrations below pathogenic levels, opening the door to new prophylactic approaches against American foulbrood and real-time epidemiological studies.

Original languageEnglish
Pages (from-to)195-201
Number of pages7
JournalInternational Journal of Food Microbiology
Volume81
Issue number3
DOIs
Publication statusPublished - 25 Mar 2003
Externally publishedYes

Keywords

  • American foulbrood
  • Apis mellifera
  • Honeybee
  • Paenibacillus larvae
  • PCR

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