Rapid determination of acetaminophen and p-aminophenol in pharmaceutical formulations using miniaturized capillary electrophoresis with amperometric detection

Qingcui Chu, Lianmei Jiang, Xiuhui Tian, Jiannong Ye*

*Corresponding author for this work

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Capability of fast analysis of a novel miniaturized capillary electrophoresis with carbon disk electrode amperometric detection (mini-CE-AD) system was demonstrated by determining acetaminophen and p-aminophenol in dosage forms. Factors influencing the separation and detection processes were examined and optimized. Under the optimum conditions, the end-capillary 300 μm carbon disc electrode amperometric detector offered favorable signal-to-noise characteristics at a relatively low potential (+600 mV versus Ag/AgCl) for detecting acetaminophen and p-aminophenol. Two analytes can been separated within 150 s in a 8.5 cm length capillary at a separation voltage of 2000 V using a Na2B4O7-KH2PO4 running buffer (pH 7.2). Acetaminophen and p-aminophenol could be detected down to the 1.4 × 10-6-5.9 × 10-7 mol L-1 level with linearity up to the 1.0 × 10-3 mol L-1 level examined. The inter-day repeatability for analytes in peak current (R.S.D. ≤ 2.3%) and migration times (R.S.D. ≤ 1.3%) were excellent. The proposed mini-CE-AD system should find a wide range of analytical applications in pharmaceutical formulations as an alternative to conventional CE and μ-CE.

Original languageEnglish
Pages (from-to)246-251
Number of pages6
JournalAnalytica Chimica Acta
Volume606
Issue number2
DOIs
Publication statusPublished - 14 Jan 2008
Externally publishedYes

Keywords

  • Acetaminophen
  • Amperometric detection
  • Capillary electrophoresis
  • Miniaturization
  • p-Aminophenol

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