逐级提取-高效液相色谱法快速测定植物组织中8种有机酸

Translated title of the contribution: Rapid determination of eight organic acids in plant tissue by sequential extraction and high performance liquid chromatography

Tianzhi Huang, Shijie Wang*, Xiuming Liu, Hong Liu, Yanyou Wu, Xuqiang Luo

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A sequential extraction method was developed to determine different forms of oxalate and seven oxalate-metabolism-related organic acids (glyoxylic acid, tartaric acid, gly-colic acid, malic acid, acetic acid, citric acid, succinic acid) in plant tissue. The ultra-pure water was used as the extraction medium to obtain water-soluble oxalic acid and the other seven water-soluble organic acids. After the extraction of the water-soluble organic acids, the resi-dues were extracted by dilute hydrochloric acid successively to get the acid-soluble oxalate which entered the liquid phase. A Hypersil ODS column was used with 5 mmol / L potassium dihydrogen phosphate buffer solution (pH 2.8) as the mobile phase. The diode array detector was set at 210 nm and the column temperature at 30 °C with the injection volume of 5 μL. The flow rate was controlled at different times which allowed a good and rapid separation of the organic acids and hydrochloric acid. Under these conditions, the linear ranges of the method were 1-2 000 mg / L for oxalic acid, 25-2 000 mg / L for acetic acid, and 10-2 000 mg / L for glyoxylic acid, tartaric acid, glycolic acid, malic acid, citric acid and succinic acid, with the correlation coefficients of the eight organic acids ≥ 0.999 6. The average recoveries of the eight organic acids in leaves and roots were 93.5% -104.4% and 85.3% -105.4% with RSDs of 0.15% -2.43% and 0.31% -2.9% (n =7), respectively. The limits of detection ranged from 1 to 10 ng (S / N = 3). The results indicated that the method is accurate, rapid and reproducible for the determination of organic acids in plant samples.

Translated title of the contributionRapid determination of eight organic acids in plant tissue by sequential extraction and high performance liquid chromatography
Original languageCantonese
Pages (from-to)1356-1361
Number of pages6
JournalChinese Journal of Chromatography (Se Pu)
Volume32
Issue number12
DOIs
Publication statusPublished - Dec 2014
Externally publishedYes

Keywords

  • High performance liquid chromatography (HPLC)
  • Organic acids
  • Plant
  • Sequential extraction

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