Rapid extraction of PCR-Competent DNA from recalcitrant environmental samples

Michael R. Gillings*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Citations (Scopus)


Advances in sequencing technologies have made the investigation of microbial ecology and community dynamics more tractable. The critical first step in such analyses is the efficient and representative recovery of PCR-competent DNA from complex environmental samples. All extraction protocols contain inherent biases, meaning that choice of method involves compromise between various factors, including efficiency, yield, universality, and representative extraction. Here, details are given for a routine method used in our laboratory to extract DNA from soils, sediments, biofilms, roots, and fungi.

Original languageEnglish
Title of host publicationEnvironmental Microbiology
Subtitle of host publicationMethods and Protocols
EditorsIan T. Paulsen, Andrew J. Holmes
Place of PublicationTotowa, NJ
PublisherHumana Press
Number of pages7
ISBN (Electronic)9781627037129
ISBN (Print)9781627037112
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology: Methods and Protocols
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Biofilm
  • Microbial diversity
  • Microbial ecology
  • PCR
  • Sediment
  • Soil

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