Rapid extraction of PCR-Competent DNA from recalcitrant environmental samples

Michael R. Gillings*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapter

    14 Citations (Scopus)


    Advances in sequencing technologies have made the investigation of microbial ecology and community dynamics more tractable. The critical first step in such analyses is the efficient and representative recovery of PCR-competent DNA from complex environmental samples. All extraction protocols contain inherent biases, meaning that choice of method involves compromise between various factors, including efficiency, yield, universality, and representative extraction. Here, details are given for a routine method used in our laboratory to extract DNA from soils, sediments, biofilms, roots, and fungi.

    Original languageEnglish
    Title of host publicationEnvironmental Microbiology
    Subtitle of host publicationMethods and Protocols
    EditorsIan T. Paulsen, Andrew J. Holmes
    Place of PublicationTotowa, NJ
    PublisherHumana Press
    Number of pages7
    ISBN (Electronic)9781627037129
    ISBN (Print)9781627037112
    Publication statusPublished - 2014

    Publication series

    NameMethods in Molecular Biology: Methods and Protocols
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029


    • Biofilm
    • Microbial diversity
    • Microbial ecology
    • PCR
    • Sediment
    • Soil


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