Rapid extraction of PCR-Competent DNA from recalcitrant environmental samples

Michael R. Gillings*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapter

    11 Citations (Scopus)

    Abstract

    Advances in sequencing technologies have made the investigation of microbial ecology and community dynamics more tractable. The critical first step in such analyses is the efficient and representative recovery of PCR-competent DNA from complex environmental samples. All extraction protocols contain inherent biases, meaning that choice of method involves compromise between various factors, including efficiency, yield, universality, and representative extraction. Here, details are given for a routine method used in our laboratory to extract DNA from soils, sediments, biofilms, roots, and fungi.

    Original languageEnglish
    Title of host publicationEnvironmental Microbiology
    Subtitle of host publicationMethods and Protocols
    EditorsIan T. Paulsen, Andrew J. Holmes
    Place of PublicationTotowa, NJ
    PublisherHumana Press
    Pages17-23
    Number of pages7
    Edition2nd
    ISBN (Electronic)9781627037129
    ISBN (Print)9781627037112
    DOIs
    Publication statusPublished - 2014

    Publication series

    NameMethods in Molecular Biology: Methods and Protocols
    Volume1096
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    Keywords

    • Biofilm
    • Microbial diversity
    • Microbial ecology
    • PCR
    • Sediment
    • Soil

    Fingerprint

    Dive into the research topics of 'Rapid extraction of PCR-Competent DNA from recalcitrant environmental samples'. Together they form a unique fingerprint.

    Cite this