Rapid extraction of PCR-Competent DNA from recalcitrant environmental samples

Michael R. Gillings*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

9 Citations (Scopus)

Abstract

Advances in sequencing technologies have made the investigation of microbial ecology and community dynamics more tractable. The critical first step in such analyses is the efficient and representative recovery of PCR-competent DNA from complex environmental samples. All extraction protocols contain inherent biases, meaning that choice of method involves compromise between various factors, including efficiency, yield, universality, and representative extraction. Here, details are given for a routine method used in our laboratory to extract DNA from soils, sediments, biofilms, roots, and fungi.

Original languageEnglish
Title of host publicationEnvironmental Microbiology
Subtitle of host publicationMethods and Protocols
EditorsIan T. Paulsen, Andrew J. Holmes
Place of PublicationTotowa, NJ
PublisherHumana Press
Pages17-23
Number of pages7
Edition2nd
ISBN (Electronic)9781627037129
ISBN (Print)9781627037112
DOIs
Publication statusPublished - 2014

Publication series

NameMethods in Molecular Biology: Methods and Protocols
Volume1096
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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  • Cite this

    Gillings, M. R. (2014). Rapid extraction of PCR-Competent DNA from recalcitrant environmental samples. In I. T. Paulsen, & A. J. Holmes (Eds.), Environmental Microbiology: Methods and Protocols (2nd ed., pp. 17-23). (Methods in Molecular Biology: Methods and Protocols; Vol. 1096). Totowa, NJ: Humana Press. https://doi.org/10.1007/978-1-62703-712-9_2