A new set of 15 polymorphic microsatellite markers were isolated and characterised in the Tree Dtella, Gehyra variegata. A next generation sequencing platform generated approximately 10Mbp of sequence data which contained 208 microsatellite repeats with suitable flanking region for primer design. We selected 15 microsatellite loci which were amplified by polymerase chain reaction (PCR) in 36 individuals. The number of alleles per locus ranged from 5 and 19 and observed heterozygosities from 0.14 to 0.94. We found no evidence for linkage disequilibrium or significant departure from Hardy-Weinberg expectations, making these loci suitable for population genetic analyses.