Rapid isolation of novel FK506 binding proteins from multiple organisms using gDNA and cDNA T7 phage display

Andrew M. Piggott, Alison M. Kriegel, Robert D. Willows, Peter Karuso*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    Reverse chemical proteomics using T7 phage display is a powerful technique for identifying cellular receptors of biologically active small molecules. However, to date this method has generally been limited to cDNA libraries constructed from mRNA isolated from eukaryotes. In this paper, we describe the construction of the first prokaryotic T7 phage display libraries from randomly digested Pseudomonas stutzeri and Vibrio fischeri gDNA, as well as a plant cDNA library from Arabidopsis thaliana. We also describe the use of T7 phage display to identify novel proteins from environmental DNA samples using biotinylated FK506 as a model affinity probe.

    Original languageEnglish
    Pages (from-to)6841-6850
    Number of pages10
    JournalBioorganic and Medicinal Chemistry
    Volume17
    Issue number19
    DOIs
    Publication statusPublished - 1 Oct 2009

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