Rapid purification of DNA from soil for molecular biodiversity analysis

C. Yeates; M. R. Gillings

doi:10.1046/j.1472-765X.1998.00383.x

Rapid purification of DNA from soil for molecular biodiversity analysis

C. Yeates^*, M. R. Gillings

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

97 Citations (Scopus)

Abstract

A rapid DNA extraction method utilizing a bead-beating machine is described. High molecular weight DNA could be extracted from up to 24 samples in less than 2 h. The DNA was suitable for direct use in the polymerase chain reaction (PCR). Both prokaryotic and eukaryotic cells were successfully lysed by the method, established using primers specific for these groups. The small subunits ribosomal RNA (rRNA) spacer region from both eukaryotes and eubacteria could be readily amplified with DNA from different soils generating different amplification patterns.

Original language	English
Pages (from-to)	49-53
Number of pages	5
Journal	Letters in Applied Microbiology
Volume	27
Issue number	1
DOIs	https://doi.org/10.1046/j.1472-765X.1998.00383.x
Publication status	Published - Jul 1998

Access to Document

10.1046/j.1472-765X.1998.00383.x

Cite this

@article{b5b2cd4ae40d4448918f41fbe917100a,

title = "Rapid purification of DNA from soil for molecular biodiversity analysis",

abstract = "A rapid DNA extraction method utilizing a bead-beating machine is described. High molecular weight DNA could be extracted from up to 24 samples in less than 2 h. The DNA was suitable for direct use in the polymerase chain reaction (PCR). Both prokaryotic and eukaryotic cells were successfully lysed by the method, established using primers specific for these groups. The small subunits ribosomal RNA (rRNA) spacer region from both eukaryotes and eubacteria could be readily amplified with DNA from different soils generating different amplification patterns.",

author = "C. Yeates and Gillings, {M. R.}",

year = "1998",

month = jul,

doi = "10.1046/j.1472-765X.1998.00383.x",

language = "English",

volume = "27",

pages = "49--53",

journal = "Letters in Applied Microbiology",

issn = "0266-8254",

publisher = "Blackwell Science",

number = "1",

}

TY - JOUR

T1 - Rapid purification of DNA from soil for molecular biodiversity analysis

AU - Yeates, C.

AU - Gillings, M. R.

PY - 1998/7

Y1 - 1998/7

N2 - A rapid DNA extraction method utilizing a bead-beating machine is described. High molecular weight DNA could be extracted from up to 24 samples in less than 2 h. The DNA was suitable for direct use in the polymerase chain reaction (PCR). Both prokaryotic and eukaryotic cells were successfully lysed by the method, established using primers specific for these groups. The small subunits ribosomal RNA (rRNA) spacer region from both eukaryotes and eubacteria could be readily amplified with DNA from different soils generating different amplification patterns.

AB - A rapid DNA extraction method utilizing a bead-beating machine is described. High molecular weight DNA could be extracted from up to 24 samples in less than 2 h. The DNA was suitable for direct use in the polymerase chain reaction (PCR). Both prokaryotic and eukaryotic cells were successfully lysed by the method, established using primers specific for these groups. The small subunits ribosomal RNA (rRNA) spacer region from both eukaryotes and eubacteria could be readily amplified with DNA from different soils generating different amplification patterns.

UR - http://www.scopus.com/inward/record.url?scp=0031854206&partnerID=8YFLogxK

U2 - 10.1046/j.1472-765X.1998.00383.x

DO - 10.1046/j.1472-765X.1998.00383.x

M3 - Article

AN - SCOPUS:0031854206

SN - 0266-8254

VL - 27

SP - 49

EP - 53

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 1

ER -

Rapid purification of DNA from soil for molecular biodiversity analysis

Abstract

Access to Document

Other files and links

Fingerprint

Cite this