Rapid purification of DNA from soil for molecular biodiversity analysis

C. Yeates*, M. R. Gillings

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    94 Citations (Scopus)

    Abstract

    A rapid DNA extraction method utilizing a bead-beating machine is described. High molecular weight DNA could be extracted from up to 24 samples in less than 2 h. The DNA was suitable for direct use in the polymerase chain reaction (PCR). Both prokaryotic and eukaryotic cells were successfully lysed by the method, established using primers specific for these groups. The small subunits ribosomal RNA (rRNA) spacer region from both eukaryotes and eubacteria could be readily amplified with DNA from different soils generating different amplification patterns.

    Original languageEnglish
    Pages (from-to)49-53
    Number of pages5
    JournalLetters in Applied Microbiology
    Volume27
    Issue number1
    DOIs
    Publication statusPublished - Jul 1998

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