Rate constants for reaction of hydroxyl radicals with Tris, Tricine and Hepes buffers

Mark Hicks, Janusz M. Gebicki*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

106 Citations (Scopus)

Abstract

Rate constants for the reactions of three commonly used organic buffers and hydroxyl radicals were measured using steady-state competition kinetics with thymine. For Hepes (4-(2-hydroxyethyl)-1-piperazine-ethanesulphonic acid), Tricine (N-[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]glycine and Tris (2-amino-2-hydroxymethylpropane-1,3-diol) the rate constants were 5.1×109, 1.6×109 and 1.1 × 109 1·mol-1·s-1, respectively.

Original languageEnglish
Pages (from-to)92-94
Number of pages3
JournalFEBS Letters
Volume199
Issue number1
DOIs
Publication statusPublished - 7 Apr 1986

Keywords

  • hydroxyl radical
  • hydroxyl radical scavenger
  • organic buffer
  • rate constant

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