Rational-based protein engineering: Tips and tools

Meghna Sobti, Bridget C. Mabbutt*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

1 Citation (Scopus)


The rational engineering of proteins is driven by contemporary needs for new and altered biomolecular forms. Utilizing manipulative procedures of molecular biology, it is relatively straightforward to alter protein structure and function to create mutated or fused sequences. We here give an overview of procedures and strategies for site-directed mutagenesis, construction of fusion proteins, and insertion of tags. The design of new protein constructs as well as their over-expression as recombinant products is considered. We also summarize approaches for the engineering of protein complexes by co-expression, a valuable route to generate bioactive multicomponent systems.

Original languageEnglish
Title of host publicationProtein Nanotechnology
Subtitle of host publicationProtocols, Instrumentation, and Applications
EditorsJuliet A. Gerrard
Place of PublicationNew York
PublisherHumana Press
Number of pages18
ISBN (Electronic)9781627033541
ISBN (Print)9781627033534
Publication statusPublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


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