Rational-based protein engineering: Tips and tools

Meghna Sobti, Bridget C. Mabbutt*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

    1 Citation (Scopus)

    Abstract

    The rational engineering of proteins is driven by contemporary needs for new and altered biomolecular forms. Utilizing manipulative procedures of molecular biology, it is relatively straightforward to alter protein structure and function to create mutated or fused sequences. We here give an overview of procedures and strategies for site-directed mutagenesis, construction of fusion proteins, and insertion of tags. The design of new protein constructs as well as their over-expression as recombinant products is considered. We also summarize approaches for the engineering of protein complexes by co-expression, a valuable route to generate bioactive multicomponent systems.

    Original languageEnglish
    Title of host publicationProtein Nanotechnology
    Subtitle of host publicationProtocols, Instrumentation, and Applications
    EditorsJuliet A. Gerrard
    Place of PublicationNew York
    PublisherHumana Press
    Pages233-250
    Number of pages18
    Edition2nd
    ISBN (Electronic)9781627033541
    ISBN (Print)9781627033534
    DOIs
    Publication statusPublished - 2013

    Publication series

    NameMethods in Molecular Biology
    Volume996
    ISSN (Print)1064-3745

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