Re-analysis of an original CMTX3 family using exome sequencing identifies a known BSCL2 mutation

Rabia Chaudhry*, Aditi Kidambi, Megan Hwa Brewer, Anthony Antonellis, Katherine Mathews, Garth Nicholson, Marina Kennerson

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Introduction: Charcot-Marie-Tooth (CMT) disease is a group of peripheral neuropathies affecting both motor and sensory nerves. CMTX3 is an X-linked CMT locus, which maps to chromosome Xq26.3-q27.3. Initially, CMTX3 was mapped to a 31.2-Mb region in 2 American families. We have reexamined 1 of the original families (US-PED2) by next generation sequencing. Methods: Three members of the family underwent exome sequencing. Candidate variants were validated by PCR and Sanger sequencing analysis. Conclusion: No pathogenic coding variants localizing to the CMTX3 region were identified. However, exome sequencing identified a known BSCL2 mutation (N88S). This study demonstrates the power of exome sequencing as a tool to identify gene mutations for a small family in the absence of statistically significant linkage data.

Original languageEnglish
Pages (from-to)922-924
Number of pages3
JournalMuscle and Nerve
Volume47
Issue number6
DOIs
Publication statusPublished - Jun 2013
Externally publishedYes

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