Real-time tracking of Yb3+, Tm3+ doped NaYF4 nanoparticles in living cancer cells

A. B. Kostyuk, E. L. Guryev, A. D. Vorotnov, L. M. Sencha, N. N. Peskova, E. A. Sokolova, L. Liang, V. A. Vodeneev, I. V. Balalaeva, A. V. Zvyagin

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The aim of the study was to demonstrate the possibility of real-time tracking of polyethylenimine-coated NaYF4 :Yb,Tm upconversion nanoparticles (UCNPs) in living cancer cells using wide-field microscopy technique.

Materials and Methods. Human breast adenocarcinoma SK-BR-3 cells and Yb3+ , Tm3+ doped NaYF4 nanoparticles with anti-Stokes photoluminescence were used in the study. The nanoparticles were visualized using wide-field microscope with excitation at 975 nm and signal detection in 420–842 spectral range. The analysis of the displacement of UCNPs was performed by fitting the point spread function of the photoluminescent spots corresponding to UCNP location by the Gaussian function, and calculation of mean square displacement. 

Results. UCNPs were rapidly internalized by SK-BR-3 cells and retained in the cells for at least 12 h. Two types of the particles motion were registered: (i) isotropic random spatial fluctuations with relatively small amplitudes and low rate of displacement, and (ii) flick and directional movements with rates up to 1.2 µm/s and total displacement up to tens of microns. The registered types of motion can be attributed to diffusion in local area and intracellular transport of nanoparticles encapsulated in vesicles, respectively.

Conclusion. The demonstrated tracking of UCNPs in human breast adenocarcinoma cells showed that Yb3+ , Tm3+ doped NaYF4 nanoparticles are an advanced agent for dynamic studies of intracellular processes. The implemented scheme for UCNPs tracking provides long-term observation with preservation of cell viability for at least several hours. In total, almost complete absence of cell autofluorescence and UCNPs photobleaching, low invasiveness, fast rate of image acquisition allow us to consider the proposed approach as useful for a variety of tasks in biomedical research.

LanguageEnglish
Pages57-63
Number of pages7
JournalSovremennye Tehnologii v Medicine
Volume10
Issue number1
DOIs
Publication statusPublished - 2018

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Nanoparticles
Cells
Neoplasms
Adenocarcinoma
Breast
Photobleaching
Polyethyleneimine
Image acquisition
Signal detection
Optical transfer function
Biomedical Research
Microscopy
Cell Survival
Photoluminescence
Microscopic examination
Microscopes
Observation

Keywords

  • Single-particle tracking
  • Upconversion nanoparticles
  • Wide-field microscopy

Cite this

Kostyuk, A. B., Guryev, E. L., Vorotnov, A. D., Sencha, L. M., Peskova, N. N., Sokolova, E. A., ... Zvyagin, A. V. (2018). Real-time tracking of Yb3+, Tm3+ doped NaYF4 nanoparticles in living cancer cells. Sovremennye Tehnologii v Medicine, 10(1), 57-63. https://doi.org/10.17691/stm2018.10.1.07
Kostyuk, A. B. ; Guryev, E. L. ; Vorotnov, A. D. ; Sencha, L. M. ; Peskova, N. N. ; Sokolova, E. A. ; Liang, L. ; Vodeneev, V. A. ; Balalaeva, I. V. ; Zvyagin, A. V. / Real-time tracking of Yb3+, Tm3+ doped NaYF4 nanoparticles in living cancer cells. In: Sovremennye Tehnologii v Medicine. 2018 ; Vol. 10, No. 1. pp. 57-63.
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title = "Real-time tracking of Yb3+, Tm3+ doped NaYF4 nanoparticles in living cancer cells",
abstract = "The aim of the study was to demonstrate the possibility of real-time tracking of polyethylenimine-coated NaYF4 :Yb,Tm upconversion nanoparticles (UCNPs) in living cancer cells using wide-field microscopy technique.Materials and Methods. Human breast adenocarcinoma SK-BR-3 cells and Yb3+ , Tm3+ doped NaYF4 nanoparticles with anti-Stokes photoluminescence were used in the study. The nanoparticles were visualized using wide-field microscope with excitation at 975 nm and signal detection in 420–842 spectral range. The analysis of the displacement of UCNPs was performed by fitting the point spread function of the photoluminescent spots corresponding to UCNP location by the Gaussian function, and calculation of mean square displacement. Results. UCNPs were rapidly internalized by SK-BR-3 cells and retained in the cells for at least 12 h. Two types of the particles motion were registered: (i) isotropic random spatial fluctuations with relatively small amplitudes and low rate of displacement, and (ii) flick and directional movements with rates up to 1.2 µm/s and total displacement up to tens of microns. The registered types of motion can be attributed to diffusion in local area and intracellular transport of nanoparticles encapsulated in vesicles, respectively.Conclusion. The demonstrated tracking of UCNPs in human breast adenocarcinoma cells showed that Yb3+ , Tm3+ doped NaYF4 nanoparticles are an advanced agent for dynamic studies of intracellular processes. The implemented scheme for UCNPs tracking provides long-term observation with preservation of cell viability for at least several hours. In total, almost complete absence of cell autofluorescence and UCNPs photobleaching, low invasiveness, fast rate of image acquisition allow us to consider the proposed approach as useful for a variety of tasks in biomedical research.",
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Kostyuk, AB, Guryev, EL, Vorotnov, AD, Sencha, LM, Peskova, NN, Sokolova, EA, Liang, L, Vodeneev, VA, Balalaeva, IV & Zvyagin, AV 2018, 'Real-time tracking of Yb3+, Tm3+ doped NaYF4 nanoparticles in living cancer cells', Sovremennye Tehnologii v Medicine, vol. 10, no. 1, pp. 57-63. https://doi.org/10.17691/stm2018.10.1.07

Real-time tracking of Yb3+, Tm3+ doped NaYF4 nanoparticles in living cancer cells. / Kostyuk, A. B.; Guryev, E. L.; Vorotnov, A. D.; Sencha, L. M.; Peskova, N. N.; Sokolova, E. A.; Liang, L.; Vodeneev, V. A.; Balalaeva, I. V.; Zvyagin, A. V.

In: Sovremennye Tehnologii v Medicine, Vol. 10, No. 1, 2018, p. 57-63.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Real-time tracking of Yb3+, Tm3+ doped NaYF4 nanoparticles in living cancer cells

AU - Kostyuk, A. B.

AU - Guryev, E. L.

AU - Vorotnov, A. D.

AU - Sencha, L. M.

AU - Peskova, N. N.

AU - Sokolova, E. A.

AU - Liang, L.

AU - Vodeneev, V. A.

AU - Balalaeva, I. V.

AU - Zvyagin, A. V.

PY - 2018

Y1 - 2018

N2 - The aim of the study was to demonstrate the possibility of real-time tracking of polyethylenimine-coated NaYF4 :Yb,Tm upconversion nanoparticles (UCNPs) in living cancer cells using wide-field microscopy technique.Materials and Methods. Human breast adenocarcinoma SK-BR-3 cells and Yb3+ , Tm3+ doped NaYF4 nanoparticles with anti-Stokes photoluminescence were used in the study. The nanoparticles were visualized using wide-field microscope with excitation at 975 nm and signal detection in 420–842 spectral range. The analysis of the displacement of UCNPs was performed by fitting the point spread function of the photoluminescent spots corresponding to UCNP location by the Gaussian function, and calculation of mean square displacement. Results. UCNPs were rapidly internalized by SK-BR-3 cells and retained in the cells for at least 12 h. Two types of the particles motion were registered: (i) isotropic random spatial fluctuations with relatively small amplitudes and low rate of displacement, and (ii) flick and directional movements with rates up to 1.2 µm/s and total displacement up to tens of microns. The registered types of motion can be attributed to diffusion in local area and intracellular transport of nanoparticles encapsulated in vesicles, respectively.Conclusion. The demonstrated tracking of UCNPs in human breast adenocarcinoma cells showed that Yb3+ , Tm3+ doped NaYF4 nanoparticles are an advanced agent for dynamic studies of intracellular processes. The implemented scheme for UCNPs tracking provides long-term observation with preservation of cell viability for at least several hours. In total, almost complete absence of cell autofluorescence and UCNPs photobleaching, low invasiveness, fast rate of image acquisition allow us to consider the proposed approach as useful for a variety of tasks in biomedical research.

AB - The aim of the study was to demonstrate the possibility of real-time tracking of polyethylenimine-coated NaYF4 :Yb,Tm upconversion nanoparticles (UCNPs) in living cancer cells using wide-field microscopy technique.Materials and Methods. Human breast adenocarcinoma SK-BR-3 cells and Yb3+ , Tm3+ doped NaYF4 nanoparticles with anti-Stokes photoluminescence were used in the study. The nanoparticles were visualized using wide-field microscope with excitation at 975 nm and signal detection in 420–842 spectral range. The analysis of the displacement of UCNPs was performed by fitting the point spread function of the photoluminescent spots corresponding to UCNP location by the Gaussian function, and calculation of mean square displacement. Results. UCNPs were rapidly internalized by SK-BR-3 cells and retained in the cells for at least 12 h. Two types of the particles motion were registered: (i) isotropic random spatial fluctuations with relatively small amplitudes and low rate of displacement, and (ii) flick and directional movements with rates up to 1.2 µm/s and total displacement up to tens of microns. The registered types of motion can be attributed to diffusion in local area and intracellular transport of nanoparticles encapsulated in vesicles, respectively.Conclusion. The demonstrated tracking of UCNPs in human breast adenocarcinoma cells showed that Yb3+ , Tm3+ doped NaYF4 nanoparticles are an advanced agent for dynamic studies of intracellular processes. The implemented scheme for UCNPs tracking provides long-term observation with preservation of cell viability for at least several hours. In total, almost complete absence of cell autofluorescence and UCNPs photobleaching, low invasiveness, fast rate of image acquisition allow us to consider the proposed approach as useful for a variety of tasks in biomedical research.

KW - Single-particle tracking

KW - Upconversion nanoparticles

KW - Wide-field microscopy

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U2 - 10.17691/stm2018.10.1.07

DO - 10.17691/stm2018.10.1.07

M3 - Article

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EP - 63

JO - Sovremennye Tehnologii v Medicine

T2 - Sovremennye Tehnologii v Medicine

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