Recent developments in electrochemical immunoassays and immunosensors

Jeremy M. Fowler*, Danny K Y Wong, H. Brian Halsall, William R. Heineman

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

33 Citations (Scopus)

Abstract

This chapter focuses on the various developments in electrochemical immunoassays and immunosensors after 2002. Electrochemical immunoassay is a solid phase system in which an antibody-antigen reaction takes place but the corresponding electrochemical detection is carried out elsewhere. However, an electrochemical immunosensor is a stand-alone device, with the immunoreaction and electrochemical detection occurring within the same device. There are several strategies for immobilizing a captured antibody on a solid phase, which include covalent attachment, physical adsorption, and electrostatic/physical entrapment in a polymer matrix. The (strept)avidin-biotin interaction technique is used to immobilize various types of biomolecules such as nucleic acids, polysaccharides, and proteins, including the capture antibody in immunoassay/immunosensor systems. Another commonly used affinity-based immobilization technique for capture antibodies in immunoassay systems involves a bacterial antibody-binding protein, the two most common of which are Protein A and Protein G. These proteins bind specifically to antibodies through their nonantigenic (Fc) regions, which allow the antigen binding sites of the immobilized antibody to be oriented away from the solid phase and be available to bind the target analyte. The application of conducting polymers such as polyaniline, polypyrrole, and polythiophene for immobilizing capture antibodies in immunoassay systems is widespread and they may be used in amperometric, potentiometric, and impedimetric immunoassay systems. Self-assembled monolayers (SAMs) are another attractive method for immobilizing the capture antibody in immunoassay systems, which takes advantage of the spontaneous chemisorption of alkanethiols to metals such as gold or silver to assemble highly ordered monolayers. Recently, interdigitated array (IDA) microelectrodes have gained popularity as an alternative transducer in electrochemical immunoassays in which a simple design of an IDA consists of a pair of interdigitated microelectrode fingers.

Original languageEnglish
Title of host publicationElectrochemical Sensors, Biosensors and their Biomedical Applications
EditorsXueji Zhang, Huangxian Ju, Joseph Wang
Place of PublicationAmsterdam, Boston
PublisherAcademic Press
Pages115-143
Number of pages29
Edition1st
ISBN (Print)9780123737380, 0123737389
DOIs
Publication statusPublished - 2008

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    Fowler, J. M., Wong, D. K. Y., Brian Halsall, H., & Heineman, W. R. (2008). Recent developments in electrochemical immunoassays and immunosensors. In X. Zhang, H. Ju, & J. Wang (Eds.), Electrochemical Sensors, Biosensors and their Biomedical Applications (1st ed., pp. 115-143). Amsterdam, Boston: Academic Press. https://doi.org/10.1016/B978-012373738-0.50007-6