Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola

Dhiraj Prakash Dhotre; Vaheesan Rajabal; Avinash Sharma; Girish J Kulkarni; Om Prakash; Venkata Ramana Vemuluri; Neetha Joseph; Praveen Rahi; Yogesh S. Shouche

doi:10.1099/ijsem.0.002019

Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola

Dhiraj Prakash Dhotre^*, Vaheesan Rajabal, Avinash Sharma, Girish J Kulkarni, Om Prakash, Venkata Ramana Vemuluri, Neetha Joseph, Praveen Rahi, Yogesh S. Shouche

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

The taxonomic positions of Microterricola viridarii JCM 15926^T, Phycicola gilvus DSM 18319^T and Leifsonia pindariensis JCM 15132^T were re-examined. Phylogenetic analysis and 16S rRNA gene sequence similarities revealed that all three strains are closely related with each other and form a monophyletic cluster with high sequence similarity (99.2 –99.9 %). A dendrogram constructed based on the protein spectra generated by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy also displayed close clustering of these three strains. The fatty acid profiles of three strains were very similar to each other and contained branched fatty acids (anteiso-C_15 : 0, anteiso-C_17 : 0 and iso-C_16 : 0) as the predominant cellular fatty acids. The polar lipid profiles of the three stains were similar and consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine as major polar lipids and an unknown lipid. Comparisons of morphological, chemotaxonomic and physiological data of Microterricola viridarii JCM 15926^T, Leifsonia pindariensis JCM 15132^T and Phycicola gilvus DSM 18319^T are in agreement with the features of a common genus. DNA–DNA hybridization data generated during this study showed less than 70 % reassociation value with each other indicating that they are different at species level. Based on the present study, we conclude that Phycicola gilvus DSM 18319^Tand Leifsonia pindariensis JCM 15132^T should be reclassified under the genus Microterricola, since this genus has the nomenclatural priority, and reclassified as Microterricola gilva comb. nov. (type strain SSWW-21^T=DSM 18319^T=KCTC 19185^T=JCM 30550^T) and Microterricola pindariensis comb. nov. (type strain PON10^T=LMG 24222^T=JCM 15132^T=MTCC9128^T). An emended description of the genus Microterricola is also presented.

Original language	English
Pages (from-to)	2766-2772
Number of pages	7
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	67
Issue number	8
Early online date	1 Aug 2017
DOIs	https://doi.org/10.1099/ijsem.0.002019
Publication status	Published - 2017
Externally published	Yes

Keywords

Actinomycetales/classification
Bacterial Typing Techniques
Base Composition
DNA, Bacterial/genetics
Fatty Acids
Nucleic Acid Hybridization
Phosphatidylcholines/chemistry
Phosphatidylglycerols/chemistry
Phylogeny
RNA, Ribosomal, 16S/genetics
Sequence Analysis, DNA

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Dhotre, D. P., Rajabal, V., Sharma, A., Kulkarni, G. J., Prakash, O., Vemuluri, V. R., Joseph, N., Rahi, P., & Shouche, Y. S. (2017). Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola. International Journal of Systematic and Evolutionary Microbiology, 67(8), 2766-2772. https://doi.org/10.1099/ijsem.0.002019

Dhotre, Dhiraj Prakash ; Rajabal, Vaheesan ; Sharma, Avinash et al. / Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola. In: International Journal of Systematic and Evolutionary Microbiology. 2017 ; Vol. 67, No. 8. pp. 2766-2772.

@article{38a57f20327c4dc895a49ee55e18abfb,

title = "Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola",

abstract = "The taxonomic positions of Microterricola viridarii JCM 15926T, Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T were re-examined. Phylogenetic analysis and 16S rRNA gene sequence similarities revealed that all three strains are closely related with each other and form a monophyletic cluster with high sequence similarity (99.2 –99.9 \%). A dendrogram constructed based on the protein spectra generated by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy also displayed close clustering of these three strains. The fatty acid profiles of three strains were very similar to each other and contained branched fatty acids (anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0) as the predominant cellular fatty acids. The polar lipid profiles of the three stains were similar and consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine as major polar lipids and an unknown lipid. Comparisons of morphological, chemotaxonomic and physiological data of Microterricola viridarii JCM 15926T, Leifsonia pindariensis JCM 15132T and Phycicola gilvus DSM 18319T are in agreement with the features of a common genus. DNA–DNA hybridization data generated during this study showed less than 70 \% reassociation value with each other indicating that they are different at species level. Based on the present study, we conclude that Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T should be reclassified under the genus Microterricola, since this genus has the nomenclatural priority, and reclassified as Microterricola gilva comb. nov. (type strain SSWW-21T=DSM 18319T=KCTC 19185T=JCM 30550T) and Microterricola pindariensis comb. nov. (type strain PON10T=LMG 24222T=JCM 15132T=MTCC9128T). An emended description of the genus Microterricola is also presented.",

keywords = "Actinomycetales/classification, Bacterial Typing Techniques, Base Composition, DNA, Bacterial/genetics, Fatty Acids, Nucleic Acid Hybridization, Phosphatidylcholines/chemistry, Phosphatidylglycerols/chemistry, Phylogeny, RNA, Ribosomal, 16S/genetics, Sequence Analysis, DNA",

author = "Dhotre, \{Dhiraj Prakash\} and Vaheesan Rajabal and Avinash Sharma and Kulkarni, \{Girish J\} and Om Prakash and Vemuluri, \{Venkata Ramana\} and Neetha Joseph and Praveen Rahi and Shouche, \{Yogesh S.\}",

year = "2017",

doi = "10.1099/ijsem.0.002019",

language = "English",

volume = "67",

pages = "2766--2772",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5034",

publisher = "Microbiology Society",

number = "8",

}

Dhotre, DP, Rajabal, V, Sharma, A, Kulkarni, GJ, Prakash, O, Vemuluri, VR, Joseph, N, Rahi, P & Shouche, YS 2017, 'Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola', International Journal of Systematic and Evolutionary Microbiology, vol. 67, no. 8, pp. 2766-2772. https://doi.org/10.1099/ijsem.0.002019

Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola. / Dhotre, Dhiraj Prakash; Rajabal, Vaheesan; Sharma, Avinash et al.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 67, No. 8, 2017, p. 2766-2772.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola

AU - Dhotre, Dhiraj Prakash

AU - Rajabal, Vaheesan

AU - Sharma, Avinash

AU - Kulkarni, Girish J

AU - Prakash, Om

AU - Vemuluri, Venkata Ramana

AU - Joseph, Neetha

AU - Rahi, Praveen

AU - Shouche, Yogesh S.

PY - 2017

Y1 - 2017

N2 - The taxonomic positions of Microterricola viridarii JCM 15926T, Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T were re-examined. Phylogenetic analysis and 16S rRNA gene sequence similarities revealed that all three strains are closely related with each other and form a monophyletic cluster with high sequence similarity (99.2 –99.9 %). A dendrogram constructed based on the protein spectra generated by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy also displayed close clustering of these three strains. The fatty acid profiles of three strains were very similar to each other and contained branched fatty acids (anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0) as the predominant cellular fatty acids. The polar lipid profiles of the three stains were similar and consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine as major polar lipids and an unknown lipid. Comparisons of morphological, chemotaxonomic and physiological data of Microterricola viridarii JCM 15926T, Leifsonia pindariensis JCM 15132T and Phycicola gilvus DSM 18319T are in agreement with the features of a common genus. DNA–DNA hybridization data generated during this study showed less than 70 % reassociation value with each other indicating that they are different at species level. Based on the present study, we conclude that Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T should be reclassified under the genus Microterricola, since this genus has the nomenclatural priority, and reclassified as Microterricola gilva comb. nov. (type strain SSWW-21T=DSM 18319T=KCTC 19185T=JCM 30550T) and Microterricola pindariensis comb. nov. (type strain PON10T=LMG 24222T=JCM 15132T=MTCC9128T). An emended description of the genus Microterricola is also presented.

AB - The taxonomic positions of Microterricola viridarii JCM 15926T, Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T were re-examined. Phylogenetic analysis and 16S rRNA gene sequence similarities revealed that all three strains are closely related with each other and form a monophyletic cluster with high sequence similarity (99.2 –99.9 %). A dendrogram constructed based on the protein spectra generated by matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy also displayed close clustering of these three strains. The fatty acid profiles of three strains were very similar to each other and contained branched fatty acids (anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0) as the predominant cellular fatty acids. The polar lipid profiles of the three stains were similar and consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine as major polar lipids and an unknown lipid. Comparisons of morphological, chemotaxonomic and physiological data of Microterricola viridarii JCM 15926T, Leifsonia pindariensis JCM 15132T and Phycicola gilvus DSM 18319T are in agreement with the features of a common genus. DNA–DNA hybridization data generated during this study showed less than 70 % reassociation value with each other indicating that they are different at species level. Based on the present study, we conclude that Phycicola gilvus DSM 18319T and Leifsonia pindariensis JCM 15132T should be reclassified under the genus Microterricola, since this genus has the nomenclatural priority, and reclassified as Microterricola gilva comb. nov. (type strain SSWW-21T=DSM 18319T=KCTC 19185T=JCM 30550T) and Microterricola pindariensis comb. nov. (type strain PON10T=LMG 24222T=JCM 15132T=MTCC9128T). An emended description of the genus Microterricola is also presented.

KW - Actinomycetales/classification

KW - Bacterial Typing Techniques

KW - Base Composition

KW - DNA, Bacterial/genetics

KW - Fatty Acids

KW - Nucleic Acid Hybridization

KW - Phosphatidylcholines/chemistry

KW - Phosphatidylglycerols/chemistry

KW - Phylogeny

KW - RNA, Ribosomal, 16S/genetics

KW - Sequence Analysis, DNA

UR - https://www.scopus.com/pages/publications/85029504322

U2 - 10.1099/ijsem.0.002019

DO - 10.1099/ijsem.0.002019

M3 - Article

C2 - 28857730

SN - 1466-5034

VL - 67

SP - 2766

EP - 2772

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 8

ER -

Dhotre DP, Rajabal V, Sharma A, Kulkarni GJ, Prakash O, Vemuluri VR et al. Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola. International Journal of Systematic and Evolutionary Microbiology. 2017;67(8):2766-2772. Epub 2017 Aug 1. doi: 10.1099/ijsem.0.002019

Reclassification of Phycicola gilvus (Lee et al. 2008) and Leifsonia pindariensis (Reddy et al. 2008) as Microterricola gilva comb. nov. and Microterricola pindariensis comb. nov. and emended description of the genus Microterricola

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Keywords

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