Recombinant human antibodies: linkage of an Fab fragment from a combinatorial library to an Fc fragment for expression in mammalian cell culture

Eckhard Bender*, Jennifer M. Woof, Julie D. Atkin, Mike D. Barker, Chris R. Bebbington, Dennis R. Burton

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

The combinatorial phage library approach to immunoglobulin repertoire cloning recently made it possible to isolate gene fragments encoding human immunoglobulin G1 Fabs binding with high affinity to specific antigens. Here we describe the construction of genes encoding whole human anti–tetanus toxoid antibodies based on one of these gene fragments and the efficient expression of these constructs by co-transfection of separate heavy and light chain vectors into a Chinese hamster ovary cell line constitutively expressing a viral transactivator protein. This system will be generally useful for the rapid analysis of recombinant antibodies derived from combinatorial libraries.

Original languageEnglish
Pages (from-to)74-79
Number of pages6
JournalHuman Antibodies
Volume4
Issue number2
DOIs
Publication statusPublished - 1993
Externally publishedYes

Keywords

  • Cho cells
  • Human antibody
  • Immunoglobulin repertoire cloning
  • Tetanus toxoid

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