Reconstitution of dendritic and natural killer-cell subsets after allogeneic stem cell transplantation: Effects of endogenous flt3 ligand

Elena Chklovskaia, Pegah Nowbakht, Catherine Nissen, Alois Gratwohl, Mario Bargetzi, Aleksandra Wodnar-Filipowicz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

72 Citations (Scopus)

Abstract

Recovery of dendritic cells (DCs) and natural killer (NK) cells after allogeneic stem cell transplantation (SCT) is important for allograft responses and antitumor immunity and thus for treatment outcome. Regulation of this regenerative process is not well understood. We investigated the influence of endogenous cytoldnes on the recovery and diversification of DC and NK cell subsets up to 6 months after SCT. Reconstitution of circulating DCs and NK cells was rapid but accompanied by prolonged skewing of cell subsets. The speed of recovery of CD11c+ CD123low DC1 exceeded that of CD11c- CD123+ DC2, and correlated with plasma levels of flt3 ligand (FL), but not with granulocyte or granulocyte-macrophage colony-stimulating factors and stem cell factor. There was a 5-fold increase in interferon-γ-producing CD56high CD16-/low NK cells and a corresponding reduction in the CD56low CD16high subset, accompanied by strongly reduced NK cell cytotoxicity. In vitro data implicate ah inhibitory effect of cyclosporin A on NK cell differentiation and cytotoxicity. NK cell numbers did not correlate with plasma levels of FL or Interleukin 15. Our results demonstrate that endogenous FL has distinct effects on the kinetics of reconstitution of DCs and NK cells and have potential implications for the modulation of Immune responses after allogeneic SCT.

Original languageEnglish
Pages (from-to)3860-3868
Number of pages9
JournalBlood
Volume103
Issue number10
DOIs
Publication statusPublished - 15 May 2004
Externally publishedYes

Fingerprint

Dive into the research topics of 'Reconstitution of dendritic and natural killer-cell subsets after allogeneic stem cell transplantation: Effects of endogenous flt3 ligand'. Together they form a unique fingerprint.

Cite this